Hattori Koji, Sugiura Shinji, Kanamori Toshiyuki
1Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
J Lab Autom. 2013 Dec;18(6):437-45. doi: 10.1177/2211068213503155. Epub 2013 Sep 6.
Cell-based assays are widely used in the various stages of drug discovery. Advances in microfluidic systems over the past two decades have enabled them to become a powerful tool for cell-based assays to achieve both reliability and high throughput. The interface between the micro-world and macro-world is important in industrial assay processes. Therefore, microfluidic cell-based assays using pressure-driven liquid handling are an ideal platform for integrated assays. The aim of this article is to review recent advancements in microfluidic cell-based assays focusing on a pressure-driven perfusion culture device. Here, we review the development of microfluidic cell-based assay devices and discuss the techniques involved in designing a microfluidic network, device fabrication, liquid and cell manipulation, and detection schemes for pressure-driven perfusion culture devices. Finally, we describe recent progress in semiautomatic and reliable pressure-driven microfluidic cell-based assays.
基于细胞的分析方法在药物发现的各个阶段都有广泛应用。过去二十年来微流控系统的进步使其成为基于细胞分析以实现可靠性和高通量的强大工具。微观世界与宏观世界之间的界面在工业分析过程中很重要。因此，使用压力驱动液体处理的基于微流控细胞的分析是集成分析的理想平台。本文的目的是回顾基于微流控细胞的分析的最新进展，重点是压力驱动灌注培养装置。在此，我们回顾基于微流控细胞的分析装置的发展，并讨论设计微流控网络、装置制造、液体和细胞操作以及压力驱动灌注培养装置检测方案所涉及的技术。最后，我们描述了半自动且可靠的基于压力驱动微流控细胞分析的最新进展。
