Moghaddam Mostafa, Naghi Amirali, Hassani Fatemeh, Amini Sedighe
Department of Immunohematology, Central Lab. of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.
Asian J Transfus Sci. 2013 Jul;7(2):156-7. doi: 10.4103/0973-6247.115584.
Alloimmunization against the Rhesus-D (RhD) antigen still remains as a major cause of hemolytic disease of fetus and newborn (HDFN). Determination of paternal RhDzygosity is performed by molecular testing and is valuable for the management of alloimmunized pregnant women. A 30-year-old pregnant woman with AB negative blood group presented with two consecutive abortions and no history of blood transfusion. By application of the antibody screening, identification panel, and selected cells, she was found to be highly alloimmunized. RhDzygosity was performed on her partner and was shown to be homozygous for RhD. The sequence- specific priming-polymerase chain reaction used in this report is essential to establish whether the mother requires an appropriate immunoprophylaxis or the fetus is at risk of HDFN.
针对恒河猴-D（RhD）抗原的同种免疫仍然是胎儿和新生儿溶血病（HDFN）的主要原因。通过分子检测确定父亲的RhD纯合性，这对于管理同种免疫的孕妇很有价值。一名30岁的AB型阴性血孕妇出现两次连续流产，且无输血史。通过抗体筛查、鉴定板和选定细胞检测，发现她处于高度同种免疫状态。对其伴侣进行了RhD纯合性检测，结果显示为RhD纯合子。本报告中使用的序列特异性引物聚合酶链反应对于确定母亲是否需要适当的免疫预防或胎儿是否有患HDFN的风险至关重要。
