Hyun Min Woo, Kim Ji Hye, Suh Dong Yeon, Lee Seung Kyu, Kim Seong Hwan
Department of Microbiology, Dankook University, Cheonan, Chungnam 330-714, Korea.
Mycobiology. 2007 Sep;35(3):159-61. doi: 10.4489/MYCO.2007.35.3.159. Epub 2007 Sep 30.
Pine tree death caused by pine wood nematode (PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle (JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. From JPS adult beetles, nine genera of Aspergillus, Gibberella, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidiomycetes were found. Ten genera from PWN-infected wood were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectrtia, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.
松材线虫（PWN）导致的松树死亡涉及松材线虫与其传播媒介日本松墨天牛（JPS）之间的携带关系。为了了解参与松材线虫生命周期的真菌多样性，从松材线虫、日本松墨天牛的成虫和幼虫以及2005年在韩国晋州砍伐的患松材线虫病的日本黑松中总共收集了176株真菌分离物。基于显微镜观察、菌落形态以及ITS rDNA的序列分析，在属的水平上鉴定了这些真菌分离物。从松材线虫中鉴定出包括毛霉属、长喙壳属和青霉属在内的三个属。从日本松墨天牛幼虫中发现了长喙壳属和青霉属两个属。从日本松墨天牛成虫中，发现了曲霉属、赤霉属、肉座菌属、耙齿菌属、小球腔菌属、长喙壳属、青霉属、束球壳属九个属以及未知的担子菌。从感染松材线虫的木材中确认了10个属，分别是双革菌属、葡萄孢属、卡马孢属、镰刀菌属、肉座菌属、丛赤壳属、毛霉属、长喙壳属、青霉属和木霉属。青霉属和长喙壳属在松材线虫及其传播媒介和寄主上普遍分布。这是韩国关于与松材线虫及其传播媒介和寄主相关真菌的首次报道。
