通过聚合酶链反应（PCR）快速检测染色木材中的云杉长喙壳菌和栎长喙壳菌。
Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
作者信息
Kim S H, Uzunovic A, Breuil C
机构信息
Chair of Forest Products Biotechnology, Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
出版信息
Appl Environ Microbiol. 1999 Jan;65(1):287-90. doi: 10.1128/AEM.65.1.287-290.1999.
Abstract
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.
摘要
开发了一种快速、灵敏且简单的方法来检测染色木材中的污斑真菌云杉长喙壳菌和栎长喙壳菌。通过使用微波加热提取DNA，并使用内转录间隔区衍生的特异性引物进行PCR，即使从单个分生孢子座获得的DNA，也能在4小时内完成检测。该方法可轻松扩展用于检测其他木材栖息真菌。
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