Park Sang-Hyun, Eom Ahn-Heum
Department of Biology Education, Korea National University of Education, Chungbuk 363-791, Korea.
Mycobiology. 2007 Dec;35(4):186-90. doi: 10.4489/MYCO.2007.35.4.186. Epub 2007 Dec 31.
This study was conducted to investigate the effects of foliar endophytic fungi and arbuscular mycorrhizal fungi (AMF) on plant community structure in experimental microcosms containing an assemblage of five species of plants (Oenothera odorata, Plantago asiatica, Trifolium repens, Isodon japonicas and Aster yomena). Leaves of Sasa borealis, Potentilla fragarioides, and Viola mandshurica were collected in Chungbuk, Korea. Endophytic fungi were isolated from the surface sterilized leaves and identified to species level using molecular and morphological techniques. Four isolates of the endophytic fungi were inoculated to the leaves of host plants in the microcosms. Also, three species of AMF spores were extracted from pure cultures and the mixture of the three species inoculated to the roots of the plants. After four months of growth in a green house, effects of both symbiotic fungi on plant species diversity, community composition and productivity were examined. The plant species diversity showed significant differences with inoculation of the symbiotic fungi. Results indicate that AMF significantly affect plant productivity and plant community structure.
本研究旨在调查叶内生真菌和丛枝菌根真菌（AMF）对含有五种植物（月见草、车前草、白三叶草、夏枯草和紫菀）组合的实验微宇宙中植物群落结构的影响。在韩国忠北采集了青篱竹、草莓委陵菜和东北堇菜的叶子。从表面消毒的叶子中分离内生真菌，并使用分子和形态学技术将其鉴定到物种水平。将四种内生真菌分离株接种到微宇宙中宿主植物的叶子上。此外，从纯培养物中提取三种AMF孢子，并将这三种孢子的混合物接种到植物的根部。在温室中生长四个月后，研究了两种共生真菌对植物物种多样性、群落组成和生产力的影响。接种共生真菌后，植物物种多样性显示出显著差异。结果表明，AMF显著影响植物生产力和植物群落结构。
