Rejinold N Sanoj, Baby Thejus, Nair Shantikumar V, Jayakumar R
Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
J Biomed Nanotechnol. 2013 Oct;9(10):1657-71. doi: 10.1166/jbn.2013.1657.
The study aims at the targeted imaging using CdTe/ZnTe core shell QDs and delivery of paclitaxel (PTX) loaded fibrinogen coated yellow-QDs (PTX-fib-yellow-QDs) towards breast cancer cells via the alpha5Beta1-integrins. We developed fibrinogen coated different sized CdTe/ZnTe core shell quantum dots of 2-10 nm size, which have been prepared by one-pot aqueous-phase approach. The fib-coated-QDs (fib-coated-QDs) and PTX-fib-yellow-QDs were prepared by two-step coacervation technique using CaCl2 as cross-linker. Particle size of fib-coated-QDs was in between 60-220 nm while PTX-fib-yellow-QDs showed 180 +/- 40 nm. The MTT assay confirmed cytocompatibility of fib-coated-QDs on L929 and MCF-7 than bare QDs, whereas significant toxicity toward MCF-7 by PTX-fib-yellow-QDs was observed. The hemocompatible fib-coated-QDs showed enhanced localization and retention toward alpha5beta1-integrins +ve MCF-7 compared to alpha5beta1-integrins -ve L929 cells. The specific binding of fib-coated-yellow-QDs was further confirmed with alpha5beta1-integrins +ve HeLa and alpha5/beta1-integrins -ve HT29 cells. Cellular uptake studies revealed localization of PTX-fib-coated-yellow-QDs inside MCF-7 cells compared to the normal L929 cells. These results indicated that fib-coated-QDs could be used for targeted imaging and as a suitable "nanocarrier" aiming breast cancer cells.
该研究旨在利用碲化镉/碲化锌核壳量子点进行靶向成像，并通过α5β1整合素将负载紫杉醇（PTX）的纤维蛋白原包被的黄色量子点（PTX-fib-黄色-QDs）递送至乳腺癌细胞。我们开发了纤维蛋白原包被的尺寸为2-10nm的不同大小的碲化镉/碲化锌核壳量子点，这些量子点是通过一锅水相法制备的。纤维蛋白原包被的量子点（fib-coated-QDs）和PTX-fib-黄色-QDs是使用氯化钙作为交联剂通过两步凝聚技术制备的。fib-coated-QDs的粒径在60-220nm之间，而PTX-fib-黄色-QDs的粒径为180±40nm。MTT试验证实，与裸量子点相比，fib-coated-QDs对L929和MCF-7细胞具有细胞相容性，而PTX-fib-黄色-QDs对MCF-7细胞具有显著毒性。与α5β1整合素阴性的L929细胞相比，具有血液相容性的fib-coated-QDs对α5β1整合素阳性的MCF-7细胞表现出更强的定位和滞留。fib-coated-黄色-QDs与α5β1整合素阳性的HeLa细胞和α5/β1整合素阴性的HT29细胞的特异性结合进一步得到证实。细胞摄取研究表明，与正常的L929细胞相比，PTX-fib-包被的黄色量子点在MCF-7细胞内有定位。这些结果表明，fib-coated-QDs可用于靶向成像，并作为一种合适的“纳米载体”用于靶向乳腺癌细胞。
