The Molecular Foundry, Lawrence Berkeley National Laboratory , 1 Cyclotron Road, Berkeley, California 94720, United States.
ACS Nano. 2013 Oct 22;7(10):9276-86. doi: 10.1021/nn403899y. Epub 2013 Sep 18.
The ability of antibodies to bind a wide variety of analytes with high specificity and high affinity make them ideal candidates as molecular recognition elements for chemical and biological sensors. However, their widespread use in sensing devices has been hampered by their poor stability and high production cost. Here we report the design and synthesis of a new class of antibody-mimetic materials based on functionalized peptoid nanosheets. A high density of conformationally constrained peptide and peptoid loops are displayed on the surface of free-floating nanosheets to generate an extended, multivalent two-dimensional material that is chemically and biologically stable. The nanosheet serves as a robust, high-surface area scaffold upon which to display a wide variety of functional loop sequences. The functionalized nanosheets were characterized by atomic force microscopy, X-ray diffraction, and X-ray reflectivity measurements, and were shown to serve as substrates for enzymes (protease and casein kinase II), as well as templates for the growth of defined inorganic materials (gold metal).
抗体能够与各种分析物结合，具有高特异性和高亲和力，这使得它们成为化学和生物传感器中分子识别元件的理想候选物。然而，由于其稳定性差和生产成本高，它们在传感设备中的广泛应用受到了阻碍。在这里，我们报告了一类基于功能化肽纳米片的新型抗体模拟材料的设计和合成。在自由漂浮的纳米片表面展示高密度的构象受限肽和肽环，以生成扩展的、多价的二维材料，该材料具有化学和生物稳定性。纳米片作为一个坚固的、高表面积的支架，可在其上展示各种功能环序列。通过原子力显微镜、X 射线衍射和 X 射线反射率测量对功能化纳米片进行了表征，并证明它们可用作酶（蛋白酶和酪蛋白激酶 II）的底物，以及用于生长特定无机材料（金金属）的模板。
