Zhang Hai-lin, Fu Shi-hong, Deng Zhang, Yuan Jun, Jiang Hong-yue, Li Ming-hua, Gao Xiao-yan, Wang Jing-lin, Liu Yong-hua, Yin Zheng-liu, Yang Wei-hong, Zhang Yu-zhen, Feng Yun, Wang Huan-yu, Liang Guo-dong
Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2013 May;34(5):428-32.
To understand the epidemiologic characteristics of dengue fever, imported from Myanmar to the border of Yunnan province, China. Viral molecular epidemiologic features were also studied.
Questionnaires were used on each diagnosed, suspected dengue fever, case or unknown cases with fever when coming from Myanmar entering the port and hospitals in Ruili city of Yunnan province. Serum samples of these patients were collected to detect IgM antibody against dengue virus and RT-PCR assay. Homology and phylogenetic tree based on the whole nucleotide sequence of PrM-C and NS5 gene of dengue virus were further analyzed.
A total of 103 sera were collected from patients at acute stage in Ruili city in July to November 2008. Among them, 49 cases were confirmed for dengue fever according to IgM and nucleic acid testings. Except one, other 48 cases were all imported into Ruili, from Myanmar. Of those, 18 patients were residents from Mujie city of Myanmar and hospitalized in Ruili and the rest 30 patients were Chinese citizens who had finished business and returned from Myanmar. Two isolates of serum samples from the imported cases were identified and both homology and phylogenetic analysis were performed, using the nucleotide sequences of PrM and NS5 genes. They were divided into dengue type 1 (RLB61) and dengue type 3 (RLC31) and were closer to the dengue virus strains isolated from Southeast Asia countries.
It is confirmed that an epidemic of dengue fever which was imported from Myanmar to Ruili city of Yunnan province, China. Evidence also showed that both type I and III epidemic strains of dengue virus did exist in Mujie city of Myanmar in 2008.
了解从缅甸输入中国云南省边境的登革热流行病学特征，并研究病毒分子流行病学特征。
对每例来自缅甸进入口岸及云南省瑞丽市医院诊断为登革热、疑似登革热或不明原因发热的病例进行问卷调查。采集这些患者的血清样本检测登革病毒IgM抗体并进行RT-PCR检测。进一步分析基于登革病毒PrM-C和NS5基因全核苷酸序列的同源性及系统发育树。
2008年7月至11月在瑞丽市共采集急性期患者血清103份。其中，49例经IgM和核酸检测确诊为登革热。除1例本地感染病例外，其余48例均为从缅甸输入瑞丽。其中，18例为缅甸木姐市居民，在瑞丽住院，其余30例为从缅甸经商回国的中国公民。对2份输入病例血清样本进行病毒分离鉴定，并对PrM和NS5基因核苷酸序列进行同源性及系统发育分析，分别分型为登革1型（RLB61）和登革3型（RLC31），且与东南亚国家分离的登革病毒株亲缘关系较近。
证实云南省瑞丽市存在从缅甸输入的登革热疫情，且2008年缅甸木姐市存在登革病毒Ⅰ型和Ⅲ型流行毒株。
