Ferrara J J, Dyess D L, Luterman A, Curreri P W
Department of Surgery, University of South Alabama, Mobile.
J Burn Care Rehabil. 1990 Jul-Aug;11(4):281-6. doi: 10.1097/00004630-199007000-00002.
Serum of patients with severe burns has the ability to suppress mitogen-induced lymphocyte proliferation. Edema fluid collected from the site of injury during resuscitation and at the time of fascial excision has also been shown to suppress this response. An animal model was designed to identify the onset time of the formation of suppressive factors, their potential site(s) of origin, and their route of entry into the systemic circulation. Femoral artery, saphenous vein, and lateral saphenous lymphatic vessels were cannulated in an anesthetized dog. Baseline samples were collected, the hind paw was subjected to scald injury, and samples were collected serially for up to 6 hours after burn injury. Tissue fluid beneath the scald injury was harvested at the end of the experiments. In the absence of significant serologic evidence for inhibition, lymph and tissue fluid exhibited early and prolonged suppression of mitogen-induced lymphocyte proliferation. We concluded that edema fluid capable of cell-mediated immune suppression forms early after thermal injury and that lymph generated from the site of burn injury is immunosuppressive.
严重烧伤患者的血清具有抑制丝裂原诱导的淋巴细胞增殖的能力。复苏期间以及筋膜切除时从损伤部位收集的水肿液也已显示出可抑制这种反应。设计了一种动物模型来确定抑制因子形成的起始时间、其潜在的起源部位以及它们进入体循环的途径。在一只麻醉的狗身上插入股动脉、大隐静脉和外侧大隐淋巴管。采集基线样本，后爪遭受烫伤，烧伤后连续采集样本长达6小时。实验结束时收集烫伤损伤下方的组织液。在没有明显抑制的血清学证据的情况下，淋巴液和组织液对丝裂原诱导的淋巴细胞增殖表现出早期和持续的抑制作用。我们得出结论，能够介导细胞免疫抑制的水肿液在热损伤后早期形成，并且烧伤部位产生的淋巴液具有免疫抑制作用。
