Deitch E A, Xu D Z, Qi L
Department of Surgery, Louisiana State University Medical Center, Shreveport 71130-3932.
Ann Surg. 1990 Jan;211(1):72-7. doi: 10.1097/00000658-199001000-00013.
Because of the association between the development of an immunocompromised state and an increased risk of infection, increasing attention has been focused on describing and characterizing the immune consequences of thermal injury. Results of human studies are largely based on the in vitro responsiveness of peripheral blood leukocytes, while splenocytes are generally used in the animal studies. Because the response of lymphocytes from different lymphocyte compartments may vary, we compared the responses of murine peripheral blood, splenic, Peyer's patch, and mesenteric lymph node lymphocytes to a battery of mitogens after thermal injury. Burn-induced immunosuppression was maximal in the splenic lymphocyte compartment, where the responses to all three test mitogens were depressed throughout the 28-day postburn study period. Although the PHA-induced mitogen response of lymphocytes from the other three lymphoid compartments remained suppressed throughout the study period, the response to the mitogens Con-A and PWM generally returned to normal or supranormal levels by the seventh postburn day, Therefore it appears that the effect of a thermal injury on lymphocyte function varies according to the lymphocyte compartment examined and the mitogen tested. These results raise the question of whether animal studies using splenic lymphocytes can be correlated with human studies performed on circulating blood lymphocytes.
由于免疫功能低下状态的发展与感染风险增加之间存在关联，人们越来越关注描述和表征热损伤的免疫后果。人体研究结果主要基于外周血白细胞的体外反应性，而动物研究中一般使用脾细胞。由于来自不同淋巴细胞区室的淋巴细胞反应可能不同，我们比较了热损伤后小鼠外周血、脾脏、派尔集合淋巴结和肠系膜淋巴结淋巴细胞对一系列促有丝分裂原的反应。烧伤诱导的免疫抑制在脾淋巴细胞区室中最为明显，在烧伤后28天的研究期间，对所有三种测试促有丝分裂原的反应均受到抑制。尽管在整个研究期间，来自其他三个淋巴区室的淋巴细胞对PHA诱导的促有丝分裂原反应仍受到抑制，但对促有丝分裂原Con-A和PWM的反应通常在烧伤后第7天恢复到正常或超正常水平。因此，热损伤对淋巴细胞功能的影响似乎因所检测的淋巴细胞区室和所测试的促有丝分裂原而异。这些结果提出了一个问题，即使用脾淋巴细胞的动物研究是否能与对循环血淋巴细胞进行的人体研究相关联。
