College of Animal Science and Technology, Shandong Agricultural University, Shandong Engineering Research Center for Animal Disease Control and Prevention, Taian 271018, China.
Vaccine. 2013 Nov 4;31(46):5479-85. doi: 10.1016/j.vaccine.2013.08.072. Epub 2013 Sep 7.
To develop an inactivated vaccine against subgroup B avian leukosis virus (ALV-B) and determine if vaccination of chicken breeders could protect young chicks from ALV-B horizontal infection at early stage and accelerate eradication progress.
Chicken embryo fibroblast (CEF) cells were inoculated with SDAU09C2 strain of ALV-B and ALV-CEF was inactivated for preparation of oil-adjuvant vaccine. Eggs were collected from un-vaccinated and 9 vaccinated great parent female chickens for incubation. 1-day-old chicks were bled for testing their maternal antibodies to ALV-A/B and then inoculated with ALV-B. Viremia and cloaca p27 detection dynamics were tested and compared between chick groups with or without maternal antibody to ALV.
In 3 weeks after 3 vaccination with the inactivated vaccine, all 9 vaccinated breeders developed high antibody titers against ALV-A/B with ELISA read values of 1.69-1.89 (the positive base line was 0.4) and kept at the high titers for at least another 4 weeks. Maternal antibody was detected in 70% (12/17) of chicks from breeders with high antibody titers to ALV-A/B. Only 4 of 12 chickens with maternal antibodies developed temporary viremia and no viremia was detected in the left 8 maternal antibody positive chickens during the whole 14 week after inoculation of ALV-B at 1 day of age. But the persistent viremia was detected in 2-8 weeks in all 9 maternal antibody negative chickens and the viremia persisted in the whole tested period of 14 weeks after inoculation of ALV-B.
The inactivated ALV-B vaccine could induce high titer antibody reaction to ALV-B, it could provide maternal antibodies to 1-day-old chickens and protect chickens from early infection of ALV-B.
研制针对禽白血病病毒亚群 B（ALV-B）的灭活疫苗，并研究种鸡免疫该疫苗能否保护雏鸡免受 ALV-B 的水平感染，以及能否促进该病的净化进程。
用 SDAU09C2 株 ALV-B 感染鸡胚成纤维细胞（CEF），制备 ALV-CEF 油乳剂灭活疫苗。采集未免疫和免疫的 9 只祖代母鸡的种蛋进行孵化，1 日龄雏鸡采血检测其针对 ALV-A/B 的母源抗体，然后接种 ALV-B。检测比较有无母源抗体的雏鸡组的病毒血症和泄殖腔 p27 检出动态。
免疫灭活疫苗 3 周后，9 只免疫种鸡均产生针对 ALV-A/B 的高抗体滴度，ELISA 检测值为 1.69-1.89（阳性基线为 0.4），至少在接下来的 4 周内保持高滴度。在母源抗体滴度高的种鸡所产的 17 只雏鸡中，有 70%（12/17）检测到母源抗体。12 只母源抗体阳性鸡中有 4 只出现短暂的病毒血症，在接种 ALV-B 后 1 天至 14 周的整个过程中，其余 8 只母源抗体阳性鸡均未检测到病毒血症。而在 9 只母源抗体阴性鸡中，所有鸡在 2-8 周均检测到持续的病毒血症，在接种 ALV-B 后 14 周的整个检测期内，病毒血症持续存在。
ALV-B 灭活疫苗可诱导机体产生针对 ALV-B 的高滴度抗体反应，能为 1 日龄雏鸡提供母源抗体，保护雏鸡免受早期 ALV-B 感染。
