Naganuma H, Ohtani H, Harada N, Nagura H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
J Histochem Cytochem. 1990 Oct;38(10):1427-32. doi: 10.1177/38.10.2401783.
In this study we investigated the immunohistochemical localization of a unique aromatase, a single protein of 51,000 daltons, in the human placenta and ovary at light and electron microscopic levels. Microwave fixation was adopted for the immunoelectron microscopic study because it is an excellent method for preserving antigenicity and subcellular structures in frozen sections. Tissue samples from four immature human placentas, four full-term human placentas, and two human ovaries fixed in 10% formalin were examined by light microscopy. In addition, tissues from three full-term human placentas and one immature human placenta fixed in 4% paraformaldehyde were examined by electron microscopy. By light microscopy, immunoreactivity for this aromatase was located in the syncytiotrophoblast and a part of the cytotrophoblast of the placenta and in the lutein and granulosa cells of the ovary. Immunoelectron microscopy revealed that the aromatase antigen was localized on the surface of the microvilli, the lateral plasma membrane, and in the endoplasmic reticulum (ER) in the syncytiotrophoblast of the placenta. The positive immunoreactivity in the syncytiotrophoblast ER is consistent with previous results using antibodies for other types of aromatase, whereas the reactivity on the microvilli has not been previously described. The present report describes the fine localization of this unique aromatase in placental and ovarian tissues; its localization on the plasma membranes requires further physiological investigation.
在本研究中，我们在光学显微镜和电子显微镜水平上研究了一种独特的芳香化酶（一种分子量为51,000道尔顿的单一蛋白质）在人胎盘和卵巢中的免疫组织化学定位。免疫电子显微镜研究采用微波固定，因为它是在冰冻切片中保存抗原性和亚细胞结构的极佳方法。对取自4个未成熟人胎盘、4个足月人胎盘以及2个用10%福尔马林固定的人卵巢的组织样本进行光学显微镜检查。此外，对取自3个足月人胎盘和1个用4%多聚甲醛固定的未成熟人胎盘的组织进行电子显微镜检查。通过光学显微镜观察，这种芳香化酶的免疫反应性位于胎盘的合体滋养层和部分细胞滋养层以及卵巢的黄体细胞和颗粒细胞中。免疫电子显微镜显示，芳香化酶抗原定位于胎盘合体滋养层的微绒毛表面、侧质膜以及内质网中。合体滋养层内质网中的阳性免疫反应性与先前使用针对其他类型芳香化酶的抗体所得到的结果一致，而微绒毛上的反应性此前尚未见报道。本报告描述了这种独特的芳香化酶在胎盘和卵巢组织中的精细定位；其在质膜上的定位需要进一步的生理学研究。
