McCarthy K J, Couchman J R
Department of Cell Biology, University of Alabama, Birmingham 35294.
J Histochem Cytochem. 1990 Oct;38(10):1479-86. doi: 10.1177/38.10.2401786.
Heparan sulfate proteoglycans have been described as the major proteoglycan component of basement membranes. However, previous investigators have also provided evidence for the presence of chondroitin sulfate glycosaminoglycan in these structures. Recently we described the production and characterization of core protein-specific monoclonal antibodies (MAb) against a chondroitin sulfate proteoglycan (CSPG) present in Reichert's membrane, a transient extra-embryonic structure of rodents. This CSPG was also demonstrated to be present in adult rat kidney. We report here the tissue distribution of epitopes recognized by these MAb. The ubiquitous presence of these epitopes in the basement membranes of nearly all adult rat tissues demonstrates that at least one CSPG is a constituent of most basement membranes, and by virtue of its unique distribution is distinct from other chondroitin and dermatan sulfate proteoglycans previously described.
硫酸乙酰肝素蛋白聚糖被认为是基底膜的主要蛋白聚糖成分。然而，先前的研究人员也提供了证据，证明这些结构中存在硫酸软骨素糖胺聚糖。最近，我们描述了针对硫酸软骨素蛋白聚糖（CSPG）的核心蛋白特异性单克隆抗体（MAb）的制备和特性，该硫酸软骨素蛋白聚糖存在于啮齿动物的一种短暂的胚外结构——雷氏膜中。这种CSPG在成年大鼠肾脏中也有发现。我们在此报告这些单克隆抗体所识别的表位的组织分布。这些表位在几乎所有成年大鼠组织的基底膜中普遍存在，这表明至少一种CSPG是大多数基底膜的组成成分，并且由于其独特的分布，与先前描述的其他硫酸软骨素和硫酸皮肤素蛋白聚糖不同。
