Holland S M, Gaydos C A, Quinn T C
Division of Infectious Diseases, Johns Hopkins Hospital, Baltimore, MD 21205.
J Infect Dis. 1990 Oct;162(4):984-7. doi: 10.1093/infdis/162.4.984.
The polymerase chain reaction was used to detect major outer membrane protein (MOMP) gene sequences from the three species of Chlamydia. Using three primer pairs and one restriction enzyme digestion, three distinct genotypes, corresponding to the three species, Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci, were demonstrated. C. trachomatis was amplified by all three primer pairs and the amplified fragment was digested by EcoRI. C. pneumoniae was amplified by only two of the three primer pairs, and the amplified fragment was digested by EcoRI. C. psittaci was amplified by only two of the pairs and the amplified fragment was EcoRI-resistant. C. trachomatis was detected in direct patient specimens, tissue culture specimens, and fixed specimens, and all serovars of C. trachomatis were detectable. The polymerase chain reaction can detect and differentiate the three species of Chlamydia and may prove a valuable diagnostic tool.
采用聚合酶链反应检测三种衣原体的主要外膜蛋白（MOMP）基因序列。使用三对引物和一种限制性酶切，证明了与沙眼衣原体、肺炎衣原体和鹦鹉热衣原体这三种衣原体相对应的三种不同基因型。沙眼衣原体可被所有三对引物扩增，扩增片段可被EcoRI酶切。肺炎衣原体仅被三对引物中的两对扩增，扩增片段可被EcoRI酶切。鹦鹉热衣原体仅被两对引物扩增，扩增片段对EcoRI具有抗性。在直接取自患者的标本、组织培养标本和固定标本中均检测到了沙眼衣原体，并且沙眼衣原体的所有血清型均可被检测到。聚合酶链反应能够检测并区分这三种衣原体，可能是一种有价值的诊断工具。
