Gaydos C A, Palmer L, Quinn T C, Falkow S, Eiden J J
Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21205-2196.
Int J Syst Bacteriol. 1993 Jul;43(3):610-2. doi: 10.1099/00207713-43-3-610.
The 16S ribosomal DNA sequence of Chlamydia pneumoniae was determined and compared with the corresponding gene sequences of Chlamydia psittaci and Chlamydia trachomatis. C. pneumoniae has been reported to exhibit little chromosomal DNA homology with the other chlamydial species, and its phylogenetic relationships within the genus Chlamydia have not been described. A polymerase chain reaction was employed to determine the 16S rRNA gene sequence of C. pneumoniae. Ten primers from the C. psittaci sequences were used to amplify a C. pneumoniae template in overlapping segments of the gene. Sequence data for 1,554 bases indicated that the levels of homology of C. pneumoniae with C. psittaci and C. trachomatis were 96.19 and 94.07%, respectively. These data support the results of previous biochemical and developmental studies indicating that C. pneumoniae is more closely related to C. psittaci than to C. trachomatis.
测定了肺炎衣原体的16S核糖体DNA序列，并将其与鹦鹉热衣原体和沙眼衣原体的相应基因序列进行比较。据报道，肺炎衣原体与其他衣原体物种的染色体DNA同源性较低，其在衣原体属内的系统发育关系尚未见描述。采用聚合酶链反应来测定肺炎衣原体的16S rRNA基因序列。使用来自鹦鹉热衣原体序列的10个引物，在基因的重叠片段中扩增肺炎衣原体模板。1554个碱基的序列数据表明，肺炎衣原体与鹦鹉热衣原体和沙眼衣原体的同源性水平分别为96.19%和94.07%。这些数据支持了先前生化和发育研究的结果，表明肺炎衣原体与鹦鹉热衣原体的关系比与沙眼衣原体的关系更为密切。
