Kaltenboeck B, Kousoulas K G, Storz J
Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
J Bacteriol. 1993 Jan;175(2):487-502. doi: 10.1128/jb.175.2.487-502.1993.
DNA sequences coding for 81% of the ompA gene from 24 chlamydial strains, representing all chlamydial species, were determined from DNA amplified by polymerase chain reactions. Chlamydial strains of serovars and strains with similar chromosomal restriction fragment length polymorphism had identical ompA DNA sequences. The ompA sequences were segregated into 23 different ompA alleles and aligned with each other, and phylogenetic relationships among them were inferred by neighbor-joining and maximum parsimony analyses. The neighbor-joining method produced a single phylogram which was rooted at the branch between two major clusters. One cluster included all Chlamydia trachomatis ompA alleles (trachoma group). The second cluster was composed of three major groups of ompA alleles: psittacosis group (alleles MN, 6BC, A22/M, B577, LW508, FEPN, and GPIC), pneumonia group (Chlamydia pneumoniae AR388 with the allele KOALA), and polyarthritis group (ruminant and porcine chlamydial alleles LW613, 66P130, L71, and 1710S with propensity for polyarthritis). These groups were distinguished through specific DNA sequence signatures. Maximum parsimony analysis yielded two equally most parsimonious phylograms with topologies similar to the ompA tree of neighbor joining. Two phylograms constructed from chlamydial genomic DNA distances had topologies identical to that of the ompA phylogram with respect to branching of the chlamydial species. Human serovars of C. trachomatis with essentially identical genomes represented a single taxonomic unit, while they were divergent in the ompA tree. Consistent with the ompA phylogeny, the porcine isolate S45, previously considered to be Chlamydia psittaci, was identified as C. trachomatis through biochemical characteristics. These data demonstrate that chlamydial ompA allelic relationships, except for human serovars of C. trachomatis, are cognate with chromosomal phylogenies.
通过聚合酶链反应扩增的DNA，测定了代表所有衣原体种类的24株衣原体ompA基因81%的编码DNA序列。血清型衣原体菌株以及具有相似染色体限制性片段长度多态性的菌株具有相同的ompA DNA序列。将ompA序列分为23个不同的ompA等位基因并相互比对，通过邻接法和最大简约法分析推断它们之间的系统发育关系。邻接法产生了一个单一的系统树图，其根位于两个主要类群之间的分支处。一个类群包括所有沙眼衣原体ompA等位基因（沙眼组）。第二个类群由三个主要的ompA等位基因组组成：鹦鹉热组（等位基因MN、6BC、A22/M、B577、LW508、FEPN和GPIC）、肺炎组（肺炎衣原体AR388及其等位基因考拉）和多关节炎组（反刍动物和猪衣原体等位基因LW613、66P130、L71和1710S，有引发多关节炎的倾向）。这些类群通过特定的DNA序列特征得以区分。最大简约法分析产生了两个同样最简约的系统树图，其拓扑结构与邻接法的ompA树相似。由衣原体基因组DNA距离构建的两个系统树图在衣原体种类的分支方面具有与ompA系统树图相同的拓扑结构。基因组基本相同的沙眼衣原体人类血清型代表一个单一的分类单元，而它们在ompA树中是不同的。与ompA系统发育一致，先前被认为是鹦鹉热衣原体的猪分离株S45通过生化特征被鉴定为沙眼衣原体。这些数据表明，除了沙眼衣原体人类血清型外，衣原体ompA等位基因关系与染色体系统发育是同源的。
