Słoczyńska Karolina, Kózka Mariusz, Marona Henryk
Department of Bioorganic Chemistry, Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Krakow, Poland.
Postepy Hig Med Dosw (Online). 2013 Jul 24;67:690-4. doi: 10.5604/17322693.1059670.
Red blood cells' (RBC) rheological properties are disturbed in chronic venous disease (CVD). The aim of the study was to compare deformability and aggregation of erythrocytes taken from the varicose vein and the antecubital vein of patients with chronic venous disease.
Blood samples were taken from twelve CVD patients presenting clinical, aetiological, anatomical and pathological elements (CEAP) stages II and III. Blood was sampled from varicose veins and antecubital veins of patients (as control). Deformability and aggregation of RBC were analysed with a Laser-assisted Optical Rotational Cell Analyser (LORCA).
A significant increase in deformability was found in varicose vein RBC for shear stress values 4.24, 8.23 and 15.96 Pa as compared to RBC from the antecubital vein. The aggregation index was significantly lower and aggregation halftime was significantly increased for RBC taken from antecubital veins than for RBC from varicose veins.
In conclusion, RBC taken from varicose and antecubital veins of CVD patients are not entirely rheologically comparable and show different deformability and aggregation. Varicose vein RBC are more deformable and show a higher tendency for aggregation than antecubital vein RBC. Perhaps the deformability of varicose vein RBC has been increased as a compensation mechanism in subjects with CVD, due to increased resistance in their microcirculation.
慢性静脉疾病（CVD）中红细胞（RBC）的流变学特性会受到干扰。本研究的目的是比较慢性静脉疾病患者曲张静脉和肘前静脉中红细胞的变形性和聚集性。
从12例处于临床、病因、解剖和病理要素（CEAP）II期和III期的CVD患者采集血样。从患者的曲张静脉和肘前静脉采集血液（作为对照）。使用激光辅助光学旋转细胞分析仪（LORCA）分析红细胞的变形性和聚集性。
与肘前静脉的红细胞相比，曲张静脉红细胞在剪切应力值为4.24、8.23和15.96 Pa时，变形性显著增加。肘前静脉采集的红细胞的聚集指数显著低于曲张静脉采集的红细胞，且聚集半衰期显著延长。
总之，CVD患者曲张静脉和肘前静脉采集的红细胞在流变学上并不完全可比，且显示出不同的变形性和聚集性。曲张静脉红细胞比肘前静脉红细胞更易变形，且聚集倾向更高。或许由于CVD患者微循环阻力增加，曲张静脉红细胞的变形性增加是一种代偿机制。
