Maeda Megumi, Akiyama Tsuyoshi, Yokouchi Daisuke, Woo Kwan Kit, Kimura Yoshinobu
Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University.
Biosci Biotechnol Biochem. 2013;77(9):1973-6. doi: 10.1271/bbb.130303. Epub 2013 Sep 7.
The β-xylosidase, which is active against plant complex type N-glycans, was purified to homogeneity from Ginkgo biloba seeds. The N-terminal amino acid sequence, G-S-A-A-G-N-R-, of the Ginkgo β-xylosidase (β-Xyl'ase Gb) was consistent with the deduced internal amino acid sequence of an Arabidopsis β-xylosidase (AtBXL1). β-Xyl'ase Gb hydrolyzed the β1-2 xylosyl residue from Xylβ1-2Manβ1-4GlcNAcβ1-4GlcNAc-PA and Xylβ1-2Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA, but not that from Manα1-6(Manα1-3)(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc-PA.
从银杏种子中纯化出了对植物复合型N - 聚糖具有活性的β - 木糖苷酶，使其达到了同质状态。银杏β - 木糖苷酶（β - Xyl'ase Gb）的N端氨基酸序列G - S - A - A - G - N - R - ，与拟南芥β - 木糖苷酶（AtBXL1）推导的内部氨基酸序列一致。β - Xyl'ase Gb能从Xylβ1 - 2Manβ1 - 4GlcNAcβ1 - 4GlcNAc - PA和Xylβ1 - 2Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA中水解β1 - 2木糖基残基，但不能从Manα1 - 6(Manα1 - 3)(Xylβ1 - 2)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 3)GlcNAc - PA中水解该残基。
