Kirik O V, Korzhevskiy D E
Northwestern Branch Institute of Experimental Medicine, RAMS, St. Petersburg, Russia
Morfologiia. 2013;143(3):71-3.
The aim of the study was to test the hypothesis on the presence, in intact rat brain, of the cells located outside the ependymal layer, but possessing the structural organization and cytochemical characteristics similar to those of ependymocytes. The study was carried out on Wistar rats (n=10). Ependymocytes were identified using immunocytochemical reactions to ezrin and vimentin and were visualized with light and confocal microscopy. Cells, structurally and cytochemically similar to typical ependymocytes, were found outside the layer of ependymocytes in the nervous tissue of intact rat brain. It is suggested that extraependymal ependymocytes may have a function of reserve population of the neural stem cells in the brain.
本研究的目的是验证一个假设,即在完整大鼠脑中,存在位于室管膜层之外,但具有与室管膜细胞相似的结构组织和细胞化学特征的细胞。该研究以Wistar大鼠(n = 10)为对象进行。通过针对埃兹蛋白和波形蛋白的免疫细胞化学反应鉴定室管膜细胞,并利用光学显微镜和共聚焦显微镜进行观察。在完整大鼠脑的神经组织中,在室管膜细胞层之外发现了结构和细胞化学上与典型室管膜细胞相似的细胞。提示室管膜外室管膜细胞可能具有作为脑内神经干细胞储备群体的功能。