Kolos Ye A, Korzhevskiy D E
Northwestern Branch Institute of Experimental Medicine, RAMS, St. Petersburg, Russia
Morfologiia. 2013;143(3):74-6.
The aim of this research was to study the expression of vimentin, one of the intermediate filament proteins, and S100 protein in the cells of developing dorsal root ganglion in prenatal ontogenesis in Wistar rat embryos (n=22) on 12-19 days of development and in newborn rats (n=6). Immunohistochemical staining methods were used to study the formation of rat dorsal root ganglion glial cells. Vimentin-immunopositive neural and glial progenitor cells were present in dorsal root ganglion primordia in rat embryos at 12-17 prenatal days. At the later stages (day 19 of prenatal life and day 1 of postnatal life) vimentin was found to be synthesized only by the differentiating glial cells. It was shown that at the same time, S100 protein was first detected in the glial cells of rat dorsal root ganglion. On postnatal day 1, the tendency was noted for the accumulation of S100 protein in the larger neurons, that was characteristic to mature dorsal root ganglia.
本研究旨在研究波形蛋白（一种中间丝蛋白）和S100蛋白在Wistar大鼠胚胎（n = 22）发育12 - 19天以及新生大鼠（n = 6）产前发育过程中背根神经节细胞中的表达。采用免疫组织化学染色方法研究大鼠背根神经节胶质细胞的形成。波形蛋白免疫阳性的神经和胶质祖细胞存在于产前12 - 17天大鼠胚胎的背根神经节原基中。在后期阶段（产前第19天和产后第1天），发现波形蛋白仅由分化的胶质细胞合成。结果表明，与此同时，S100蛋白首次在大鼠背根神经节的胶质细胞中被检测到。在出生后第1天，观察到S100蛋白在较大神经元中积累的趋势，这是成熟背根神经节的特征。
