Petrova E S, Isaeva E N, Korzhevskii D E
Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology, Research Institute of Experimental Medicine, North-West Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia,
Bull Exp Biol Med. 2014 Sep;157(5):637-40. doi: 10.1007/s10517-014-2633-7. Epub 2014 Sep 26.
The development of dissociated cells from rat embryonic spinal ganglion after transplantation to damaged nerve of adult animals was studied using immunohistochemical differentiation markers of neural and glial cells. The cell suspension obtained after dissociation of rat embryonic spinal ganglia (embryonic day 15) was injected into the proximal segment of crushed sciatic nerve. The nerve was damaged by ligation for 40 sec. Progenitor cells were labeled with 5-bromo-2'-deoxyuridine (BrdU) before transplantation. BrdU-immunopositive cells were detected in the nerve trunks of recipients on days 1, 21, and 28 after transplantation. Dissociated cells of rat embryonic spinal ganglion (embryonic day 15) survived for at least 4 weeks after transplantation to the nerve and differentiate into NeuN-immunopositive neurons with morphological properties of sensory neurons and satellite cells containing S100 protein.
利用神经和胶质细胞的免疫组织化学分化标志物，研究了大鼠胚胎脊髓神经节解离细胞移植到成年动物受损神经后的发育情况。将大鼠胚胎脊髓神经节（胚胎第15天）解离后获得的细胞悬液注入坐骨神经挤压伤的近端。神经通过结扎40秒造成损伤。移植前用5-溴-2'-脱氧尿苷（BrdU）标记祖细胞。在移植后第1天、第21天和第28天，在受体的神经干中检测到BrdU免疫阳性细胞。大鼠胚胎脊髓神经节（胚胎第15天）的解离细胞移植到神经后存活至少4周，并分化为具有感觉神经元形态特征的NeuN免疫阳性神经元和含有S100蛋白的卫星细胞。
