Laboratory of Immunosenescence, Institute of Biomedical Research, Pontifical Catholic University of the Rio Grande do Sul (PUCRS), Porto Alegre.
Bipolar Disord. 2013 Dec;15(8):832-8. doi: 10.1111/bdi.12121. Epub 2013 Sep 10.
Bipolar disorder (BD) has been associated with persistent low-grade inflammation and premature cell senescence, as shown by reduced telomere length (TL). The human cytomegalovirus (CMV) has increasingly been implicated in accelerated immunosenescence in aging studies. Here, we compared CMV serology and its relationships with cell senescence markers, including TL and lymphocyte subsets, in patients with type I BD and healthy controls.
Twenty-two euthymic female patients with BD type I and 17 age-matched healthy controls were selected for the study. A sample of blood was collected and mononuclear cells and DNA were isolated and TL measured. CMV immunoglobulin M (IgM) and IgG titers were measured using chemiluminescent assays. Lymphocyte subsets [T, natural killer (NK) and NKT] were phenotyped by flow cytometry.
Individuals with BD had shorter TLs but higher CMV IgG levels than controls (both p < 0.01). CMV IgG level was inversely correlated with TL. None of the subjects showed IgM reactivity for CMV, excluding acute viral infection. CMV IgG level was associated with expansion of senescent CD8+CD28- T cells and NK cells, which are involved in viral control.
These data support the hypothesis of accelerated aging in BD, as shown by shortened telomeres, higher seropositivity for CMV, and expansion of senescent T cells.
双相情感障碍（BD）与持续低度炎症和细胞早衰有关，表现为端粒长度（TL）缩短。人类巨细胞病毒（CMV）在衰老研究中越来越被认为与免疫衰老加速有关。在这里，我们比较了 CMV 血清学及其与细胞衰老标志物（包括 TL 和淋巴细胞亚群）的关系，这些标志物在 I 型 BD 患者和健康对照者中。
选择 22 名病情稳定的女性 I 型 BD 患者和 17 名年龄匹配的健康对照者进行研究。采集血样，分离单核细胞和 DNA，测量 TL。使用化学发光测定法测量 CMV 免疫球蛋白 M（IgM）和 IgG 滴度。通过流式细胞术对淋巴细胞亚群[T、自然杀伤（NK）和 NKT]进行表型分析。
BD 患者的 TL 较短，但 CMV IgG 水平高于对照组（均 p < 0.01）。CMV IgG 水平与 TL 呈负相关。没有受试者对 CMV 产生 IgM 反应，排除了急性病毒感染。CMV IgG 水平与衰老的 CD8+CD28-T 细胞和 NK 细胞的扩张有关，这些细胞参与病毒的控制。
这些数据支持 BD 加速衰老的假说，表现为端粒缩短、CMV 血清阳性率升高以及衰老 T 细胞的扩张。
