Liu Si-Yi, Dong Yan-Mei, Gao Xue-Jun
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2013 Apr;48(4):234-8.
To investigate the effects of bioactive glasses (BG) including 45S5 and nano-58S on proliferation, angiogenic markers vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) secretion and gene expression of human dental pulp cells (HDPC).
HDPC of 4th passage were cultured in Dulbecco's modified Eagle's medium (DMEM) which contained 0.1 g/L 45S5 or nano-58S ionic dissolution products. Meanwhile HDPC were cultured in DMEM without BG as control group. Proliferation of the cells was evaluated with methyl thiazolyl tetrazolium (MTT) assay on day 1, 2, 3. Quantitative real-time PCR and quantitative sandwich enzyme immunoassays were used to test VEGF and bFGF gene expression and protein secretion of HDPC on day 1, 2, 3.
The relative growth rate (RGR) of 45S5 and nano-58S groups were (134.5 ± 5.0)% and (146.3 ± 19.8)%, which was significantly different from that of control group (P < 0.05). The quantity of VEGF secretion of two experimental groups were (189.29 ± 4.64) and (216.18 ± 14.67) ng/L, respectively, significantly higher than that of the control group [(159.03 ± 11.69) ng/L] (P < 0.05). Furthermore, the nano-58S group secreted much more VEGF than 45S5 group (P < 0.05).bFGF secretion of HDPC was also enhanced by both 45S5 and nano-58S bioactive glasses. The VEGF gene expression of 45S5 and nano-58S on day 1 were (1.70 ± 0.19) and (1.63 ± 0.42), while the bFGF gene expressin on day 3 were (1.49 ± 0.02) and (2.30 ± 0.04), all significantly higher than that of control group (P < 0.05).
Bioactive glasses can enhance the proliferation, VEGF and bFGF secretion and gene expression of human dental pulp cells. Compared with 45S5, nano-58S showed a higher activation.
研究45S5和纳米58S等生物活性玻璃对人牙髓细胞（HDPC）增殖、血管生成标志物血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）分泌及基因表达的影响。
将第4代HDPC培养于含有0.1 g/L 45S5或纳米58S离子溶解产物的 Dulbecco改良 Eagle培养基（DMEM）中。同时，将HDPC培养于不含生物活性玻璃的DMEM中作为对照组。在第1、2、3天用甲基噻唑基四氮唑（MTT）法评估细胞增殖。在第1、2、3天，用定量实时聚合酶链反应和定量夹心酶免疫测定法检测HDPC的VEGF和bFGF基因表达及蛋白分泌。
45S5组和纳米58S组的相对生长率（RGR）分别为（134.5±5.0）%和（146.3±19.8）%，与对照组相比有显著差异（P<0.05）。两个实验组的VEGF分泌量分别为（189.29±4.64）和（216.18±14.67）ng/L，显著高于对照组[（159.03±11.69）ng/L]（P<0.05）。此外，纳米58S组分泌的VEGF比45S5组多得多（P<0.05）。45S5和纳米58S生物活性玻璃均增强了HDPC的bFGF分泌。45S5和纳米58S在第1天的VEGF基因表达分别为（1.70±0.19）和（1.63±0.42），而在第3天的bFGF基因表达分别为（1.49±0.02）和（2.30±0.04），均显著高于对照组（P<0.05）。
生物活性玻璃可增强人牙髓细胞的增殖、VEGF和bFGF分泌及基因表达。与45S5相比，纳米58S具有更高的活性。
