State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Biosens Bioelectron. 2014 Feb 15;52:69-75. doi: 10.1016/j.bios.2013.08.006. Epub 2013 Aug 26.
A novel dendritic quantum dot (QD) nanocluster was constructed and used as versatile electrochemiluminescence (ECL) and electrochemical probe for the detection of DNA and cancer cells. Owing to the many functional groups present in the nanoclusters, a large number of QDs were assembled on the nanoclusters, which could greatly amplify both the ECL and electrochemical signals of QDs. Carbon nanotubes (CNTs)/gold nanoparticles' (NPs) hybrids were used as amplified platform for assembling large numbers of DNA on the electrode, which also improve the bioactivity and stability of the electrode. After the QD-DNA signal probe was recognized with target DNA (t-DNA), the amplified ECL signal for the detection of target DNA was obtained. Furthermore, magnetic nanoparticles were employed for cell aptamers immobilization, the same QD nanocluster-DNA probe was also extended for electrochemical detection of cancer cells using sensitive anodic stripping voltammetry (ASV) method, which simplified the separation procedures and improved the sensitivity. It is anticipated that the assays could provide promising and cost effective approach for the early and accurate detection of DNA and cancer cells.
一种新型树枝状量子点（QD）纳米簇被构建并用作多功能电致化学发光（ECL）和电化学探针，用于检测 DNA 和癌细胞。由于纳米簇中存在许多官能团，因此可以在纳米簇上组装大量的 QD，这可以大大放大 QD 的 ECL 和电化学信号。碳纳米管（CNTs）/金纳米粒子（NPs）的混合物被用作在电极上组装大量 DNA 的放大平台，这也提高了电极的生物活性和稳定性。在 QD-DNA 信号探针与靶 DNA（t-DNA）识别后，获得了用于检测靶 DNA 的放大 ECL 信号。此外，还使用磁性纳米粒子进行细胞适体固定化，同样的 QD 纳米簇-DNA 探针也被扩展用于使用敏感的阳极溶出伏安法（ASV）电化学检测癌细胞，这简化了分离程序并提高了灵敏度。预计该测定方法可以为 DNA 和癌细胞的早期和准确检测提供有前途且经济有效的方法。
