Deyl Z, Butenko G M, Hausmann J, Horakova M, Macek K
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Mech Ageing Dev. 1990 Jul;55(1):39-47. doi: 10.1016/0047-6374(90)90104-n.
Changes of non-enzymatic collagen glycosylation were followed in 2- and 24-month-old rats and mice and in parabiotic animals of the same age. With advancing age increased glycation of collagen was observed in both old male Wistar rats and white mice. Further it was demonstrated that both aortal and skin collagen of young animals is rapidly non-enzymatically glycosylated in the common milieu created by parabiotic animals and the proportion of non-enzymatically incorporated glucose approaches in the young counterparts the level found in old individuals. Similar trends as with non-enzymatic glycosylation were found with a fluorescent (370/440 nm) product present in both categories of collagen preparations. This fluorescence was higher in old animals and was considerably increased in the young counterpart of the parabiotic couple 6 weeks after operation. The nature of the fluorescent product appears different from pyridinoline and remains to be elucidated.
在2月龄和24月龄的大鼠和小鼠以及相同年龄的联体动物中，对非酶促胶原糖基化的变化进行了跟踪研究。随着年龄的增长，在老年雄性Wistar大鼠和白色小鼠中均观察到胶原糖基化增加。此外，还证明了在联体动物所形成的共同环境中，幼年动物的主动脉和皮肤胶原会迅速发生非酶促糖基化，并且幼年动物中非酶促掺入葡萄糖的比例接近老年个体中的水平。在两类胶原制剂中均存在的一种荧光（370/440nm）产物，其变化趋势与非酶促糖基化相似。这种荧光在老年动物中较高，并且在联体对的幼年动物术后6周时显著增加。该荧光产物的性质似乎不同于吡啶啉，有待进一步阐明。
