基于聚乙烯亚胺的悬浮适应型HEK-293E和CHO-DG44细胞的瞬时基因表达过程
Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.
作者信息
Hacker David L, Kiseljak Divor, Rajendra Yashas, Thurnheer Sarah, Baldi Lucia, Wurm Florian M
机构信息
Protein Expression Core Facility, EPFL, CH-1015 Lausanne, Switzerland; Laboratory of Cellular Biotechnology, EPFL, CH-1015 Lausanne, Switzerland.
出版信息
Protein Expr Purif. 2013 Nov;92(1):67-76. doi: 10.1016/j.pep.2013.09.001. Epub 2013 Sep 8.
Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.
哺乳动物细胞的瞬时基因表达（TGE）是一种越来越重要的工具，可用于快速生产重组蛋白，以用于生物化学、结构生物学和生物医学等研究领域。本文综述了使用阳离子聚合物聚乙烯亚胺（PEI）进行基因传递，对悬浮培养的人胚肾（HEK-293）细胞和中国仓鼠卵巢（CHO）细胞进行转染的方法。
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