Hunt A F, Reed M I
Regional Transfusion Centre, John Radcliffe Hospital, Oxford, England, UK.
Med Lab Sci. 1990 Jul;47(3):189-94.
The binding mechanisms and binding sites involved in the tannic acid and chromic chloride-induced binding of protein to red cells were investigated using the binding of IgA paraprotein to red cells as model systems. Inhibition studies of these model systems using amino acid homopolymers and compounds (common as red cell membrane constituents) suggest that the mechanisms involved are similar to those proposed for the conversion of hide or skin collagen to leather, as in commercial tanning. These studies also suggest that tannic acid-induced binding of IgA paraprotein to red cells involves the amino acid residues of L-arginine, L-lysine, L-histidine, and L-proline analogous to tanning with phenolic plant extracts. The amino acid residues of L-aspartate, L-glutamate and L-asparagine are involved in a similar manner in chronic chloride-induced binding of protein to red cells.
以IgA副蛋白与红细胞的结合作为模型系统，研究了鞣酸和氯化铬诱导蛋白质与红细胞结合的机制及结合位点。使用氨基酸均聚物和化合物（常见的红细胞膜成分）对这些模型系统进行的抑制研究表明，其涉及的机制与商业鞣革中兽皮或皮肤胶原蛋白转化为皮革所提出的机制相似。这些研究还表明，鞣酸诱导的IgA副蛋白与红细胞的结合涉及L-精氨酸、L-赖氨酸、L-组氨酸和L-脯氨酸的氨基酸残基，类似于用酚类植物提取物鞣革。L-天冬氨酸、L-谷氨酸和L-天冬酰胺的氨基酸残基以类似方式参与氯化铬诱导的蛋白质与红细胞的结合。
