National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190 Beijing, PR China; Department of Food Science and Engineering, College of Biological Science and Biotechnology, Beijing Forestry University, 100083 Beijing, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Oct 15;937:84-90. doi: 10.1016/j.jchromb.2013.08.018. Epub 2013 Aug 18.
A simple and efficient chromatographic method for large-scale preparative separation of phenylethanoid glycosides (mainly contain echinacoside and acteoside) from Cistanche deserticola was developed. The adsorption properties of eight macroporous resins were evaluated. Three selected resins were further screened depending on the adsorption kinetics curves, in which HPD300 resin showed the best separation efficiency. The adsorption isotherm data on HPD300 resin were fitted to the Freundlich equation in certain concentration range. The dynamic adsorption and desorption experiments were carried out on columns packed with HPD300 resin to optimize the separation process. The breakthrough curves showed that acteoside had a higher affinity to the resin than echinacoside. The contents of echinacoside and acteoside in the product increased from 1.79% and 1.43% in the crude extracts to 16.66% and 15.17%, with recovery yields of 80.41% and 90.17%, respectively. The purity of total phenylethanoid glycosides in the product was 76.58%.
建立了一种从肉苁蓉中大规模制备分离苯乙醇苷类化合物（主要含有毛蕊花糖苷和麦角甾苷）的简单高效的色谱方法。评价了 8 种大孔树脂的吸附性能。根据吸附动力学曲线，进一步筛选了 3 种选定的树脂，其中 HPD300 树脂显示出最好的分离效率。在一定浓度范围内，HPD300 树脂的吸附等温线数据符合 Freundlich 方程。在装有 HPD300 树脂的柱上进行了动态吸附和解吸实验，以优化分离过程。穿透曲线表明，麦角甾苷对树脂的亲和力高于毛蕊花糖苷。产物中毛蕊花糖苷和麦角甾苷的含量从粗提物中的 1.79%和 1.43%分别增加到 16.66%和 15.17%，回收率分别为 80.41%和 90.17%。产物中总苯乙醇苷的纯度为 76.58%。
