[肉苁蓉提取物中苯乙醇苷类成分的液相色谱-电喷雾串联质谱分析]

[Analysis of phenylethanoid glycosides in the extract of herba Cistanchis by LC/ESI-MS/MS].

作者信息

Wang Y M, Zhang S J, Luo G A, Hu Y N, Hu J P, Liu L, Zhu Y, Wang H J

机构信息

Department of Chemistry, Tsinghua University, Beijing 100084, China.

出版信息

Yao Xue Xue Bao. 2000 Nov;35(11):839-42.

PMID:11218862

Abstract

AIM

To analyze the phenylethanoid glycosides in Cistanche deserticola Y. D. Ma and its alternatives.

METHODS

An HPLC/MS/MS method has been developed for the analysis of seven kinds of phenylethanoid glycosides in Cistanche deserticola Y. D. Ma, C. salsa (C. A. Mey) G. Beck and C. tubulosa (Schenk) R. Wight. The [M - H]- ions were observed for five standards and Cistanche extracts. The glycosidic linkages, the core, and the attached sugar (s) of the phenylethanoid glycosides can be determined from the collision-induced dissociation spectra of the molecular.

RESULTS

Seven kinds of phenylethanoid glycosides (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside, cistanoside A, osmanthuside B) in Cistanche deserticola Y. D. Ma, six kinds (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside and cistanoside A) in C. salsa (C. A. Mey) G. Beck and five kinds (echinacoside, acteoside, cisacteoside, isoacteoside and 2'-acetylacteoside) in C. tubulosa (Schenk) R. Wight were detected.

CONCLUSION

The difference of the relative distribution of these phenylethanoid glycosides in each extract was found out. Phenylethanoid glycosides are the specific constituents in Cistanchis, which can be used to distinguish different species in Genus Cistanchis.

摘要

目的

分析肉苁蓉及其混淆品中的苯乙醇苷类成分。

方法

建立了高效液相色谱-串联质谱法（HPLC/MS/MS），用于分析肉苁蓉、盐生肉苁蓉和管花肉苁蓉中的7种苯乙醇苷类成分。对5种对照品和肉苁蓉提取物进行负离子模式扫描，通过分子离子的二级质谱碎片信息对苯乙醇苷类成分的糖基连接位置、苷元结构及糖基取代情况进行分析。

结果

从肉苁蓉中检测出7种苯乙醇苷类成分（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷、肉苁蓉苷A、桂花苷B），从盐生肉苁蓉中检测出6种（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷、肉苁蓉苷A），从管花肉苁蓉中检测出5种（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷）。