Wang Y M, Zhang S J, Luo G A, Hu Y N, Hu J P, Liu L, Zhu Y, Wang H J
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Yao Xue Xue Bao. 2000 Nov;35(11):839-42.
To analyze the phenylethanoid glycosides in Cistanche deserticola Y. D. Ma and its alternatives.
An HPLC/MS/MS method has been developed for the analysis of seven kinds of phenylethanoid glycosides in Cistanche deserticola Y. D. Ma, C. salsa (C. A. Mey) G. Beck and C. tubulosa (Schenk) R. Wight. The [M - H]- ions were observed for five standards and Cistanche extracts. The glycosidic linkages, the core, and the attached sugar (s) of the phenylethanoid glycosides can be determined from the collision-induced dissociation spectra of the molecular.
Seven kinds of phenylethanoid glycosides (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside, cistanoside A, osmanthuside B) in Cistanche deserticola Y. D. Ma, six kinds (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside and cistanoside A) in C. salsa (C. A. Mey) G. Beck and five kinds (echinacoside, acteoside, cisacteoside, isoacteoside and 2'-acetylacteoside) in C. tubulosa (Schenk) R. Wight were detected.
The difference of the relative distribution of these phenylethanoid glycosides in each extract was found out. Phenylethanoid glycosides are the specific constituents in Cistanchis, which can be used to distinguish different species in Genus Cistanchis.
分析肉苁蓉及其混淆品中的苯乙醇苷类成分。
建立了高效液相色谱-串联质谱法（HPLC/MS/MS），用于分析肉苁蓉、盐生肉苁蓉和管花肉苁蓉中的7种苯乙醇苷类成分。对5种对照品和肉苁蓉提取物进行负离子模式扫描，通过分子离子的二级质谱碎片信息对苯乙醇苷类成分的糖基连接位置、苷元结构及糖基取代情况进行分析。
从肉苁蓉中检测出7种苯乙醇苷类成分（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷、肉苁蓉苷A、桂花苷B），从盐生肉苁蓉中检测出6种（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷、肉苁蓉苷A），从管花肉苁蓉中检测出5种（松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、异毛蕊花苷、2'-乙酰毛蕊花糖苷）。
明确了各提取物中苯乙醇苷类成分相对分布的差异。苯乙醇苷类成分是肉苁蓉属植物的特征性成分，可用于肉苁蓉属不同种的鉴别。
