Antonie Van Leeuwenhoek. 2013 Nov;104(5):779-85. doi: 10.1007/s10482-013-9987-0.
A Gram-negative, aerobic, motile by means of single polar flagellum, short rod-shaped marine bacterium, designated strain E418T, was isolated from the spines on the body surface of starfish Acanthaster planci in the Xisha islands, China. Cells of strain E418T were found to grow optimally at pH 7–8, at 25–37 °C, and in the presence of 2–5 % (w/v) NaCl. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain E418T is a member of the genus Pseudoalteromonas. The closest relative to this strain was found to be P. ruthenica LMG 19699T, with a similarity level of 97.7 %. DNA relatedness between the novel isolate and this phylogenetically related species was 57.4 %. Strain E418T decomposed Tween 80, gelatin, and casein, but was unable to decompose starch and grow on DNase Agar. The cellular fatty acid profile consisted of significant amounts of C16:1ω7c/C16:1ω6c, C18:1ω7c/C18:1ω6c, C16:0, and C17:1ω8c. The G+C content of DNA of this strain was determined to be 46.7 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that strain E418T represents a novel species of the genus Pseudoalteromonas, for which the name Pseudoalteromonas xishaensis sp. nov. is proposed. The type strain of P. xishaensis is strain E418T (DSM 25588T = NBRC 108846T = CCTCC AB 2011177T).
一株革兰氏阴性、需氧、通过单极鞭毛运动的短杆状海洋细菌，命名为 E418T 菌株，从中国西沙群岛星鱼 Acanthaster planci 体表刺中分离得到。E418T 菌株的细胞在 pH7-8、25-37°C 和 2-5%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列比较的系统发育分析表明，E418T 菌株是假性交替单胞菌属的一个成员。与该菌株最接近的是 P. ruthenica LMG 19699T，相似性水平为 97.7%。新型分离株与该系统发育相关种之间的 DNA 同源性为 57.4%。E418T 菌株分解吐温 80、明胶和酪蛋白，但不能分解淀粉和在 DNA 酶琼脂上生长。细胞脂肪酸图谱由大量 C16:1ω7c/C16:1ω6c、C18:1ω7c/C18:1ω6c、C16:0 和 C17:1ω8c 组成。该菌株的 DNA G+C 含量为 46.7 mol%。表型特征、系统发育分析和 DNA-DNA 同源性数据表明，E418T 菌株代表假性交替单胞菌属的一个新种，建议命名为假交替单胞菌西沙氏菌 sp. nov.。P. xishaensis 的模式菌株是 E418T 菌株（DSM 25588T = NBRC 108846T = CCTCC AB 2011177T）。
