Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar, 843300, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 May;103(5):997-1005. doi: 10.1007/s10482-013-9879-3. Epub 2013 Jan 13.
A novel Gram-negative, aerobic, slightly halophilic, yellow-pigmented, oxidase-negative, Voges-Proskauer positive, non-spore-forming bacterium, designated YIM M 13059(T), was isolated from a sediment sample collected from the South China Sea at a depth of 310 m. Optimal growth was found to occur at 28-30 °C, pH 7.0 and in the presence of 3-4 % (w/v) NaCl. Cells were observed to be rod-shaped and motile by peritrichous flagella. The polar lipids of strain YIM M 13059(T) were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a ninhydrin-positive phospholipid, one glycolipid and two unknown phospholipids. The predominant respiratory quinone was determined to be Q-9. The major fatty acids were identified as C18:1 ω7c, C16:1 ω6c/C16:1 ω7c, C16:0 and C12:0 3-OH. The genomic DNA G+C content was determined to be 54.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate belongs to the genus Halomonas in the family Halomonadaceae. The 16S rRNA gene sequence similarities between strain YIM M 13059 (T) and the type strains of members of the genus Halomonas were in the range 93.3-98.3 %. However, the levels of DNA-DNA relatedness values between YIM M 13059 and the type strains of the most closely related species, Halomonas zhangjiangensis, Halomonas variabilis, Halomonas neptunia, Halomonas boliviensis and Halomonas sulfadieris were 50.2 ± 0.68 %, 46.8 ± 1.9 %, 28.5 ± 0.74 %, 42.9 ± 0.55 % and 37.1 ± 0.68 %, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, the strain YIM M 13059(T) is proposed to represent a novel member of the genus Halomonas, with the name Halomonas nanhaiensis sp. nov. The type strain is YIM M 13059(T) (=JCM 18142(T) =CCTCC AB 2012911(T)).
一株新型革兰氏阴性、需氧、轻度嗜盐、黄色素产生、氧化酶阴性、Voges-Proskauer 阳性、非孢子形成的细菌，被命名为南海海洋微生物 13059(T)，从南海 310 米深处的沉积物样本中分离得到。最适生长温度为 28-30°C，pH 值为 7.0，并且需要 3-4%（w/v）的 NaCl。细胞观察到为杆状，通过周生鞭毛运动。菌株 YIM M 13059(T) 的极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种水合茚三酮阳性磷脂、一种糖脂和两种未知磷脂。主要呼吸醌为 Q-9。主要脂肪酸为 C18:1 ω7c、C16:1 ω6c/C16:1 ω7c、C16:0 和 C12:0 3-OH。基因组 DNA G+C 含量为 54.4mol%。基于 16S rRNA 基因序列的系统发育分析表明，该分离株属于海洋单胞菌科海洋单胞菌属。菌株 YIM M 13059(T)与海洋单胞菌属成员的模式株的 16S rRNA 基因序列相似性在 93.3-98.3%之间。然而，YIM M 13059 与最密切相关的物种的模式株之间的 DNA-DNA 同源性值水平分别为 50.2±0.68%、46.8±1.9%、28.5±0.74%、42.9±0.55%和 37.1±0.68%，与 Halomonas zhangjiangensis、Halomonas variabilis、Halomonas neptunia、Halomonas boliviensis 和 Halomonas sulfadieris。基于系统发育、化学分类和表型数据，菌株 YIM M 13059(T)被提议代表海洋单胞菌属的一个新型成员，命名为南海海洋单胞菌。模式株为 YIM M 13059(T)（=JCM 18142(T) =CCTCC AB 2012911(T)）。
