Tahri-Jouti M A, Chaby R
1116 du Centre National de la Recherche Scientifique, Université de Paris-Sud, Orsay, France.
Mol Immunol. 1990 Aug;27(8):751-61. doi: 10.1016/0161-5890(90)90084-d.
Tritium-labeled lipopolysaccharide interacted specifically and reversibly with mouse peritoneal macrophages. The binding was higher at 22 degrees C than at 4 degrees C, but was no longer observable at 37 degrees C. The specificity of the interaction (inhibition with unlabeled LPS) was strictly dependent on the presence of serum, and required divalent cations. The binding was saturable. The specific binding sites of peritoneal macrophages were saturated with 6-9 x 10(6) LPS molecules/cell, and those of macrophage-like cell lines with 2-3 x 10(6) molecules/cell. The binding of LPS was not inhibited by ligands of scavenger receptors (maleylated BSA) or complement receptors (zymosan), but was strongly inhibited with dexamethasone, a glucocorticoid which is known to modulate the expression of other surface markers of macrophages. The polysaccharide region of the LPS, as well as 3-deoxy-2-octulosonic acid (KDO) coupled to bovine serum albumin, did not bind to macrophages, whereas a specific binding was observed with a lipid A-BSA conjugate.
氚标记的脂多糖与小鼠腹腔巨噬细胞发生特异性且可逆的相互作用。在22℃时的结合比在4℃时更高，但在37℃时不再能观察到结合。相互作用的特异性（用未标记的脂多糖抑制）严格依赖于血清的存在，并且需要二价阳离子。结合是可饱和的。腹腔巨噬细胞的特异性结合位点被6 - 9×10⁶个脂多糖分子/细胞饱和，而巨噬细胞样细胞系的特异性结合位点被2 - 3×10⁶个分子/细胞饱和。脂多糖的结合不受清道夫受体配体（马来酰化牛血清白蛋白）或补体受体配体（酵母聚糖）的抑制，但被地塞米松强烈抑制，地塞米松是一种已知可调节巨噬细胞其他表面标志物表达的糖皮质激素。脂多糖的多糖区域以及与牛血清白蛋白偶联的3 - 脱氧 - 2 - 辛酮酸（KDO）不与巨噬细胞结合，而用脂质A - 牛血清白蛋白偶联物可观察到特异性结合。
