Tahri-Jouti M A, Mondange M, Le Dur A, Auzanneau F I, Charon D, Girard R, Chaby R
1116 du Centre National de la Recherche Scientifique, Université de Paris-Sud, Orsay, France.
Mol Immunol. 1990 Aug;27(8):763-70. doi: 10.1016/0161-5890(90)90085-e.
The interaction of lipopolysaccharide-binding sites of mouse macrophages with the Lipid A region of endotoxins (LPS) was demonstrated by direct binding of labeled Lipid A conjugates, by inhibition of the binding of labeled LPS with anti-Lipid A monoclonal antibodies, and by the considerable reduction of this binding after chemical and enzymatic removal of the fatty acid esters of the LPS. The substructures of Lipid A required for the specific binding of LPS to macrophages were analyzed by the use of synthetic lipids consisting of mono- or disaccharide derivatives of glucosamine. The two phosphate groups of Lipid A (at positions 1 and 4') as well as certain hydroxyl groups, appeared to play a critical role in the binding. However, the reactivities of the synthetic lipids with the macrophage surface, as compared with those with anti-Lipid A antibodies, could hardly be explained by the existence of a single LPS receptor, and suggested the presence, on the macrophage surface, of different LPS-binding sites that recognize different substructures or spatial configurations of the lipid moiety of endotoxins.
通过标记脂质A缀合物的直接结合、抗脂质A单克隆抗体对标记脂多糖（LPS）结合的抑制以及LPS脂肪酸酯经化学和酶法去除后该结合的显著减少，证明了小鼠巨噬细胞的脂多糖结合位点与内毒素脂质A区域（LPS）之间的相互作用。通过使用由氨基葡萄糖的单糖或二糖衍生物组成的合成脂质，分析了LPS与巨噬细胞特异性结合所需的脂质A亚结构。脂质A的两个磷酸基团（在1位和4'位）以及某些羟基似乎在结合中起关键作用。然而，与抗脂质A抗体相比，合成脂质与巨噬细胞表面的反应性很难用单一LPS受体的存在来解释，这表明巨噬细胞表面存在不同的LPS结合位点，这些位点识别内毒素脂质部分的不同亚结构或空间构型。
