Wan Lei, Liu Jian, Huang Chuan-Bing, Wang Yuan, Shen Xi, Zhang Wandong, Wang Guizheng, Fan Haixia, Ge Yao, Chen Ruilian, Cao Yunxiang, Zong Ruikai
Department of Rheumatology, The First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230031, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2013 Jul;42(4):418-25. doi: 10.3785/j.issn.1008-9292.2013.04.008.
To investigate the effects of Xinfeng Capsule (XFC) on pulmonary function and related mechanism in adjuvant-induced arthritis (AA) rats.
The rats were randomly divided into five groups: normal control (NC), model control (MC) groups, methotrexate (MTX), tripterygium glycosides tablet (TPT) and Xinfeng capsule (XFC) treatment groups. The adjuvant-induced arthritis model was established by intracutaneous injection of 0.1 mL Freund ' s complete adjuvant in the right paw of rats; the drugs were given 19 d after model establishment. The toe swelling degree (E), arthritis index (AI), pulmonary function, peripheral blood Treg levels, pathological changes of lung tissue and expression of Foxp3, TGF-β1, Smad3, Smad7 proteins in lung tissue were measured 30 d after drug administration.
Compared to NC group, the levels of E, AI, alveolitis score, TGF-β1 and Smad3 were significantly increased (P <0.05 or P <0.01); maximum expiratory flow 25% of vital capacity (FEF25),50% maximal expiratory vital capacity flow (FEF50), maximum expiratory flow at 75% of vital capacity (FEF75), maximum mid-expiratory flow (MMF), force peak expiratory flow (PEF), CD4+ CD25+ Treg, Foxp3 and Smad7 were significantly decreased in MC group (P <0.05 or P < 0.01). Compared to MC group,the expression of E, AI, TGF-β1 and Smad3 were reduced, while FEF50, FEF75, MMF, PEF, Treg, Foxp3 and Smad7 were elevated in XFC group (P <0.05 or P <0.01). Compared to XFC group, the level of body mass,FEF25,FEF50, FEF75, MMF and Treg were lower in MTX and TPT groups (P <0.05 or P <0.01).
There are inflamed joints and reduced pulmonary function in rats of adjuvant-induced arthritis. XFC can inhibit paw edema degrees, reduce arthritis response, and improve pulmonary function, which is associated with up-regulating expression of Treg and Foxp3, down-regulating the expression of TGF-β1 and adjusting TGF-β1/Smads signal pathway.
探讨新风胶囊（XFC）对佐剂性关节炎（AA）大鼠肺功能的影响及其相关机制。
将大鼠随机分为五组：正常对照组（NC）、模型对照组（MC）、甲氨蝶呤（MTX）组、雷公藤多苷片（TPT）组和新风胶囊（XFC）治疗组。通过在大鼠右后足皮内注射0.1 mL弗氏完全佐剂建立佐剂性关节炎模型；造模后19天给予药物。给药30天后，测量大鼠足趾肿胀度（E）、关节炎指数（AI）、肺功能、外周血调节性T细胞（Treg）水平、肺组织病理变化以及肺组织中叉头框蛋白3（Foxp3）、转化生长因子-β1（TGF-β1）、Smad3、Smad7蛋白的表达。
与NC组相比，MC组的E、AI、肺泡炎评分、TGF-β1和Smad3水平显著升高（P<0.05或P<0.01）；MC组的肺活量25%时的最大呼气流量（FEF25）、50%最大呼气肺活量流量（FEF50）、肺活量75%时的最大呼气流量（FEF75）、最大呼气中期流量（MMF）、用力呼气峰值流量（PEF）、CD4+CD25+Treg、Foxp3和Smad7显著降低（P<0.05或P<0.01）。与MC组相比，XFC组的E、AI、TGF-β1和Smad3表达降低，而FEF50、FEF75、MMF、PEF、Treg、Foxp3和Smad7升高（P<0.05或P<0.01）。与XFC组相比，MTX组和TPT组的体重、FEF25、FEF50、FEF75、MMF和Treg水平较低（P<0.05或P<0.01）。
佐剂性关节炎大鼠存在关节炎症和肺功能降低。新风胶囊可抑制足爪水肿程度，减轻关节炎反应，并改善肺功能，这与上调Treg和Foxp3表达、下调TGF-β1表达以及调节TGF-β1/Smads信号通路有关。
