Cheng Yuan-yuan, Liu Jian, Feng Yun-xia, Liu Lei, Wan Lei
Graduate Department, Anhui College of Traditional Chinese Medicine, Hefei, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Nov;28(11):1133-7.
To observe the effects of Xinfeng Capsule (XFC) on cardiopulmonary function of knee osteoarthritis (KOA) rats and explore its molecular mechanism.
Forty rats were randomly divided into normal control (NC) group, model control (MC) group, glucosamine control (GS) group and XFC group, 10 in each group. All rats were induced to KOA by injected papain and L-Cys into the right knee joint except NC group. Fourteen days after modeling, NC and MC groups were given normal saline (0.01 g/kg), GS and XFC groups were given glucosamine (0.098 g/kg) and XFC suspension (0.375 g/kg), respectively. Cardiac and pulmonary function were detected by ultrasonography and animal spirometry, respectively. B and T lymphocyte attenuator (BTLA), herpesvirus entry mediator (HVEM), interleukin (IL)-17, IL-4, transforming growth factor-beta (TGF-β1) were detected by ELISA; CD4(+);CD25(+);Treg and CD4(+); CD25(+);Foxp3(+); Treg were detected by flow cytometry.
Compared with NC group, body mass, levels of early diastolic peak flow velocity (E), early diastolic peak flow velocity/atrial peak flow velocity (E/A), forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), 25% forced expiratory flow (FEF25), 50% forced expiratory flow (FEF50), 75% forced expiratory flow (FEF75), maximal mid-expiratory flow (MMF), peak expiratory flow (PEF), expressions of BTLA, HVEM, IL-4, CD4(+);CD25(+);Treg and CD4(+);CD25(+);Foxp3(+); Treg significantly decreased, and TGF-β1, IL-17 significantly increased in MC group (P<0.01 or P<0.05). Compared with MC group, weight, levels of E, E/A, FEV1, FEF50, FEF75, PEF, BTLA, HVEM, IL-4, CD4(+);CD25(+);Treg and CD4(+);CD25(+);Foxp3(+); Treg significantly increased, and Mankin score, cardiacindex (CI), lung index (LI), TGF-β1, IL-17 significantly decreased in GS and XFC groups (P<0.01 or P<0.05); Compared with GS group, weight and Treg were significantly elevated in XFC group (P<0.01 or P<0.05).
XFC can decrease Mankin scores of cartilage and improve cardiopulmonary function of KOA rats. Its mechanism may be enhancing BTLA-HVEM negative co-stimulatory signals, inducing Treg immune tolerance, up-regulating IL-4, down-regulating IL-17, TGF-β1, then to inhibit abnormal inflammatory immune response.
观察新风胶囊（XFC）对膝骨关节炎（KOA）大鼠心肺功能的影响并探讨其分子机制。
将40只大鼠随机分为正常对照组（NC组）、模型对照组（MC组）、氨基葡萄糖对照组（GS组）和XFC组，每组10只。除NC组外，其余大鼠右膝关节注射木瓜蛋白酶和L-半胱氨酸诱导KOA模型。造模14天后，NC组和MC组给予生理盐水（0.01 g/kg），GS组和XFC组分别给予氨基葡萄糖（0.098 g/kg）和XFC混悬液（0.375 g/kg）。分别采用超声心动图和动物肺功能仪检测心脏和肺功能。采用酶联免疫吸附测定（ELISA）法检测B和T淋巴细胞衰减器（BTLA）、疱疹病毒进入介质（HVEM）、白细胞介素（IL）-17、IL-4、转化生长因子-β（TGF-β1）；采用流式细胞术检测CD4(+)；CD25(+)；调节性T细胞（Treg）和CD4(+)；CD25(+)；叉头框蛋白3（Foxp3）(+)；Treg。
与NC组比较，MC组体质量、舒张早期峰值流速（E）、E/心房峰值流速（E/A）、用力肺活量（FVC）、第1秒用力呼气量（FEV1）、25%用力呼气流量（FEF25）、50%用力呼气流量（FEF50）、75%用力呼气流量（FEF75）、最大呼气中期流速（MMF）、呼气峰值流速（PEF）、BTLA、HVEM、IL-4、CD4(+)；CD25(+)；Treg和CD4(+)；CD25(+)；Foxp3(+)；Treg表达显著降低，TGF-β1、IL-17显著升高（P<0.01或P<0.05）。与MC组比较，GS组和XFC组体质量、E、E/A、FEV1、FEF50、FEF75、PEF、BTLA、HVEM、IL-4、CD4(+)；CD25(+)；Treg和CD4(+)；CD25(+)；Foxp3(+)；Treg显著升高，Mankin评分、心脏指数（CI）、肺指数（LI）、TGF-β1、IL-17显著降低（P<0.01或P<0.05）；与GS组比较，XFC组体质量和Treg显著升高（P<0.01或P<0.05）。
XFC可降低软骨Mankin评分，改善KOA大鼠心肺功能。其机制可能是增强BTLA-HVEM负性共刺激信号，诱导Treg免疫耐受，上调IL-4，下调IL-17、TGF-β1，进而抑制异常炎症免疫反应。
