Schaefer U W, Schmidt C G, Dicke K A, van Bekkum D W, Schmitt G
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1975 Aug 8;83(4):285-91. doi: 10.1007/BF00573015.
The CFU-S potential of mouse bone marrow, the transplantation capacity of autologous and allogeneic monkey bone marrow and the CFU-C potential of human bone marrow can be cryopreserved without loss of viability. It is critical for an optimal stem cell recovery to avoid an osmotic shock after thawing. The intracellular cryoprotectives glycerol and DMSO which provide a high osmotic pressure should be removed from the thawed cells by a slow stepwise dilution procedure.
小鼠骨髓的集落形成单位脾（CFU-S）潜能、自体和同种异体猴骨髓的移植能力以及人骨髓的集落形成单位细胞（CFU-C）潜能均可冷冻保存而不丧失活力。为实现最佳的干细胞复苏，避免解冻后发生渗透压休克至关重要。应通过缓慢的逐步稀释程序从解冻细胞中去除提供高渗透压的细胞内冷冻保护剂甘油和二甲基亚砜（DMSO）。
