Schaefer U W, Beyer J H
Anaesthesist. 1975 Dec;24(12):505-6.
The cryoprotective effect of hydroxyethylstarch has been tested in mice and in man using the spleen colony technique (CFU-S test) or the agar colony technique (CFU-C test) as viability assays for the haemopoietic stem cell. In mice we found a mean CFU-S recovery of 88.7%+/-3.9% when a rapid, and 77.8%+/-3.2% when a slow, stepwise dilution method was applied to remove the protective medium (17.5-20% hydroxyethylstarch+20% calf serum) after thawing. In man the rapid dilution after thawing gave a mean CFU-C recovery of 80% but a wide scatter.
已使用脾集落技术（CFU-S试验）或琼脂集落技术（CFU-C试验）作为造血干细胞的活力测定方法，在小鼠和人体中测试了羟乙基淀粉的冷冻保护作用。在小鼠中，解冻后采用快速逐步稀释法去除保护培养基（17.5 - 20%羟乙基淀粉 + 20%小牛血清）时，CFU-S的平均回收率为88.7%±3.9%；采用缓慢逐步稀释法时，CFU-S的平均回收率为77.8%±3.2%。在人体中，解冻后快速稀释时CFU-C的平均回收率为80%，但离散度较大。
