Department of Virology, St. Savvas, Regional Anticancer Oncology Hospital of Athens, 171 Alexandras Avenue, 11522 Athens, Greece. +30 2106409461,
Anticancer Res. 2013 Sep;33(9):4007-11.
The goal of this study was to investigate human papillomavirus (HPV) geno typing and high-risk HPV E6/E7 mRNA expression in 849 women.
849 Cervical samples were obtained from patients attending an outpatient clinic to have their annual gynaecological check-up. All patients underwent a conventional Pap test. The patients were also offered HPV test with the knowledge that it is not part of the screening.
Overall prevalence of HPV DNA was 41.3%, while E6/E7 mRNA expression was found in 20.7% of the patients. HPV DNA and E6/E7 mRNA expression were detected in 21.1% and 9.1% of normal cytological samples, respectively, 38.1% and 23.8% of atypical squamous cells of unknown significance, 84.8% and 40.7% of low-grade squamous intraepithelial lesions, and 96.4% and 89.3% of high-grade squamous intraepithelial lesions, respectively. HPV 16 was the most frequent genotype identified.
E6/E7mRNA detection might be useful as a screening marker for the early prediction of active infections and subsequent progression to severe dysplasia.
本研究旨在调查 849 名女性的人乳头瘤病毒（HPV）基因分型和高危型 HPV E6/E7mRNA 表达。
从参加年度妇科检查的门诊患者中获得了 849 例宫颈样本。所有患者均接受了常规巴氏涂片检查。还向患者提供了 HPV 检测，他们知道这不是筛查的一部分。
HPV DNA 的总流行率为 41.3%，而 E6/E7mRNA 的表达在 20.7%的患者中被发现。在正常细胞学样本中分别检测到 HPV DNA 和 E6/E7mRNA 表达，在非典型鳞状细胞不能明确意义（ASC-US）中分别为 21.1%和 9.1%，在低级别鳞状上皮内病变（LSIL）中分别为 38.1%和 40.7%，在高级别鳞状上皮内病变（HSIL）中分别为 84.8%和 89.3%。HPV16 是最常见的基因型。
E6/E7mRNA 的检测可能作为一种筛查标志物，用于早期预测活跃感染及随后向严重发育不良的进展。
