Annika Gauss, Robert Ehehalt, Karl-Heinz Weiss, Yvonne Schaefer, Petra Kloeters-Plachky, Adolf Stiehl, Wolfgang Stremmel, Peter Sauer, Daniel Nils Gotthardt, Gastroenterology and Hepatology Unit, Department of Internal Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany.
World J Gastroenterol. 2013 Sep 7;19(33):5454-63. doi: 10.3748/wjg.v19.i33.5454.
To analyze phospholipid profiles in intrahepatic bile from patients with primary sclerosing cholangitis (PSC) and secondary sclerosing cholangitis (SSC).
Intrahepatic bile specimens collected via endoscopic retrograde cholangiography from 41 patients were analyzed. Fourteen of these patients were diagnosed with PSC, 10 with SSC, 11 with choledocholithiasis or no identifiable biliary disease, and 6 with cholangiocellular carcinoma (CCC). Bile acid, cholesterol, protein, and bilirubin contents as well as pancreas lipase activity in bile were determined by biochemical methods. Phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species were quantified using nano-electrospray ionization tandem mass spectrometry.
Bile from all the examined patient groups showed a remarkably similar PC and LPC species composition, with only minor statistical differences. Total biliary PC concentrations were highest in controls (8030 ± 1843 μmol/L) and lowest in patients with CCC (1969 ± 981 μmol/L) (P = 0.005, controls vs SSC and CCC, respectively, P < 0.05). LPC contents in bile were overall low (4.2% ± 1.8%). Biliary LPC/PC ratios and ratios of biliary PC to bilirubin, PC to cholesterol, PC to protein, and PC to bile acids showed no intergroup differences.
PC and LPC profiles being similar in patients with or without sclerosing cholangitis, these phospholipids are likely not of major pathogenetic importance in this disease group.
分析原发性硬化性胆管炎（PSC）和继发性硬化性胆管炎（SSC）患者肝内胆汁中的磷脂谱。
通过内镜逆行胰胆管造影术收集 41 名患者的肝内胆汁标本进行分析。其中 14 名患者被诊断为 PSC，10 名患者为 SSC，11 名患者为胆总管结石或无明确胆道疾病，6 名患者为胆管细胞癌（CCC）。通过生化方法测定胆汁中的胆汁酸、胆固醇、蛋白质和胆红素含量以及胰腺脂肪酶活性。采用纳喷雾电离串联质谱法定量检测磷脂酰胆碱（PC）和溶血磷脂酰胆碱（LPC）。
所有受检患者组的胆汁均显示出非常相似的 PC 和 LPC 种类组成，仅存在微小的统计学差异。总胆汁 PC 浓度在对照组中最高（8030 ± 1843 μmol/L），在 CCC 患者中最低（1969 ± 981 μmol/L）（P = 0.005，分别为对照组与 SSC 和 CCC，P < 0.05）。胆汁中的 LPC 含量总体较低（4.2% ± 1.8%）。胆汁 LPC/PC 比值以及胆汁 PC 与胆红素、PC 与胆固醇、PC 与蛋白质和 PC 与胆汁酸的比值在各组间无差异。
在有或没有硬化性胆管炎的患者中，PC 和 LPC 谱相似，这些磷脂在该疾病组中可能不是主要的发病机制重要因素。
