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[广东省市售肉类中沙门氏菌的生化及血清学特征]

[Biochemical and serological characterization Salmonella from retail meats in Guangdong Province].

作者信息

Yang Xiaojuan, Wu Qingping, Zhang Jumei, Dong Xiaohui, Liu Shengrong

机构信息

State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base), South China.

出版信息

Wei Sheng Yan Jiu. 2013 Jul;42(4):615-8.

Abstract

OBJECTIVE

Salmonella isolates from meat in Guangdong Province were characterized to determine their phenotype and serotype, to investigate contamination status of meats by Salmonella and provide scientific basis for detection and further control.

METHODS

209 meat samples were detected according to methods GB 4789.4--2010 National food safety standard Food microbiological examination: Salmonella. The API 20E system was used to identify isolates. Serotyping of Salmonella isolates was performed by slide agglutination method using antisera to Salmonella.

RESULTS

According to the detection results of 209 meat samples, forty-six Salmonella strains were isolated from 42 meat samples, the positive rate was 20. 10%. The majority of positive samples were fresh meat, 69.23% in duck, 37.14% in chicken, 20.00% in beef and 16.67% in pock. The strains identified as Salmonella by the API 20E were distributed in 7 profile numbers with two of them being predominant (6704752 and 6704552). The most common serotypes were Derby (21.74%), Typhimurium (10.87%), Enteritidis (8.70%), Tshiongwe (8.70%), Indiana (8.70%), Weltevreden (8.70%).

CONCLUSION

Salmonella present in retail meats were common and phenotypically diverse in Guangdong Province.

摘要

目的

对广东省肉类中分离出的沙门氏菌进行特征分析,以确定其表型和血清型,调查肉类受沙门氏菌污染状况,为检测及进一步防控提供科学依据。

方法

按照GB 4789.4—2010《食品安全国家标准 食品微生物学检验:沙门氏菌》方法对209份肉类样品进行检测。采用API 20E系统鉴定分离株。使用沙门氏菌抗血清通过玻片凝集法对沙门氏菌分离株进行血清分型。

结果

根据209份肉类样品的检测结果,从42份肉类样品中分离出46株沙门氏菌,阳性率为20.10%。阳性样品以鲜肉居多,鸭中占69.23%,鸡中占37.14%,牛肉中占20.00%,猪肉中占16.67%。经API 20E鉴定为沙门氏菌的菌株分布在7个谱号中,其中两个为主导谱号(6704752和6704552)。最常见的血清型为德比(21.74%)、鼠伤寒(10.87%)、肠炎(8.70%)、乔维(8.70%)、印第安纳(8.70%)、韦尔泰夫登(8.70%)。

结论

广东省市售肉类中沙门氏菌普遍存在且表型多样。

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