Tseng Li-Ping, Juan Chih-Yin, Lin Shiun-Long, Doran Michael R, Lin Jiang-Jen, Hsu Shan-Hui, Liao Jiunn-Wang, Shen Ching-I, Su Hong-Lin
Department of Life Sciences, National Chung Hsing University, Taiwan.
Nanomedicine (Lond). 2014 May;9(7):1019-33. doi: 10.2217/nnm.13.53. Epub 2013 Sep 11.
To evaluate the effectiveness of novel nanohybrids, composed of silver nanoparticles and nanoscale silicate platelets, to clear Pseudomonas aeruginosa biofilms.
MATERIALS & METHODS: The nanohybrids were manufactured from an in situ reduction of silver salts in the silicate platelet dispersion, and then applied to biofilms in vitro and in vivo.
In reference to the biocidal effects of gentamycin, the nanohybrids mitigated the spreading of the biofilms, and initiated robust cell death and exfoliation from the superficial layers of the biofilms in vitro. In vivo, the nanohybrids exhibited significant therapeutic effects by eliminating established biofilms from infected corneas and promoting the recovery of corneal integrity.
All of the evaluations indicate the high potency of the newly developed silver nanoparticle/nanoscale silicate platelet nanohybrids for eliminating biofilms.
评估由银纳米颗粒和纳米级硅酸盐薄片组成的新型纳米复合材料清除铜绿假单胞菌生物膜的有效性。
通过在硅酸盐薄片分散液中原位还原银盐制备纳米复合材料，然后将其应用于体外和体内生物膜。
与庆大霉素的杀菌效果相比，纳米复合材料减轻了生物膜的扩散，并在体外引发了生物膜表层细胞的大量死亡和脱落。在体内，纳米复合材料通过消除感染角膜上已形成的生物膜并促进角膜完整性的恢复，表现出显著的治疗效果。
所有评估表明，新开发的银纳米颗粒/纳米级硅酸盐薄片纳米复合材料具有高效清除生物膜的能力。
