Lassiani L, Ebert C, Nisi C, Varnavas A, Boccú E
Istituto di Chimica Farmaceutica e Tossicologica, Università die Trieste, Italy.
Pharmazie. 1990 Jun;45(6):414-5.
The hydrolysis of p-(3,3-dimethyl-l-triazeno)benzoic acid potassium salt (1;DM-COOK) a highly active antimetastatic and anti-disseminative agent, has been studied in buffered aqueous solution over a pH range of 2.8-8.8 degrees C. The pH dependence of the pseudo first-order rate constants showed two different routes. Under physiological conditions the hydrolysis reactions are carried out by acid catalysis. A procedure based on fourth-order derivative UV spectroscopy (D4) has been developed for the calculation of the kinetic constants at pH greater than or equal to 4.00 and no spectral interferences resulted from decomposition products. The application of derivative UV spectroscopy proved to be suitable fpr rapid, sensitive and reproducible studies of hydrolysis of this class of compounds.
对一种高活性抗转移和抗扩散剂对-（3,3-二甲基-1-三氮烯）苯甲酸钾盐（1；DM-COOK）在2.8 - 8.8℃的缓冲水溶液中的水解进行了研究。伪一级速率常数对pH的依赖性显示出两种不同途径。在生理条件下，水解反应通过酸催化进行。已开发出一种基于四阶导数紫外光谱法（D4）的程序，用于计算pH大于或等于4.00时的动力学常数，且分解产物未产生光谱干扰。事实证明，导数紫外光谱法适用于对这类化合物水解进行快速、灵敏且可重复的研究。
