Department of Obstetrics and Gynecology, Turku University Hospital, University of Turku, P.O. Box 52, 20521, Turku, Finland,
Eur J Clin Microbiol Infect Dis. 2014 Jan;33(1):123-33. doi: 10.1007/s10096-013-1938-1. Epub 2013 Sep 13.
Persistent high-risk human papillomavirus (HR-HPV) infection is the key event in the progression of HPV lesions, and more data are urgently needed on asymptomatic oral HPV infections in men. Asymptomatic fathers-to-be (n = 131, mean age 28.9 years) were enrolled in the cohort, sampled by serial oral scrapings at baseline and at 2-month, 6-month, 12-month, 24-month, 36-month, and 7-year follow-up visits to accomplish persistent and cleared HPV infections. HPV genotyping was performed using nested PCR and Multimetrix® assay. Covariates of persistent and cleared oral HPV infections were analysed using generalised estimating equation (GEE) and Poisson regression. Altogether, 17 HPV genotypes were detected in male oral mucosa point prevalence, varying from 15.1 % to 31.1 %. Genotype-specific HPV persistence was detected in 18/129 men the mean persistence time ranging from 6.0 to 30.7 months. History of genital warts decreased (p = 0.0001; OR = 0.41, 95 % CI 0.33-0.51) and smoking increased (p = 0.033, OR = 1.92, 95 % CI 1.05-3.50) the risk of persistent species 7/9 HPV infections. Of the 74 HPV-positive men, 71.6 % cleared their infection actuarial and crude clearance times, varying between 1.4 and 79.6 months. No independent predictors were identified for species 7/9 clearance. At the last follow-up-visit, 50.1 % of the fathers had oral mucosal changes, correlating only with smoking (p = 0.046). To conclude, most of the persisting oral infections in males were caused by HPV16. Smoking increased while previous genital warts decreased oral HR-HPV persistence. No predictors of HR-HPV clearance were disclosed.
持续性高危型人乳头瘤病毒（HR-HPV）感染是 HPV 病变进展的关键事件，目前迫切需要更多关于男性无症状口腔 HPV 感染的数据。本队列纳入了 131 名无症状准父亲（平均年龄 28.9 岁），他们通过基线和 2 个月、6 个月、12 个月、24 个月、36 个月和 7 年随访时的连续口腔刮片进行采样，以确定持续性和清除性 HPV 感染。采用巢式 PCR 和 Multimetrix®assay 进行 HPV 基因分型。采用广义估计方程（GEE）和泊松回归分析持续性和清除性口腔 HPV 感染的相关因素。共在男性口腔黏膜点患病率中检测到 17 种 HPV 基因型，从 15.1%到 31.1%不等。在 129 名男性中，有 18 名检测到特定基因型 HPV 持续感染，平均持续时间为 6.0 至 30.7 个月。生殖器疣病史减少（p=0.0001；OR=0.41，95%CI 0.33-0.51）和吸烟增加（p=0.033，OR=1.92，95%CI 1.05-3.50）增加了持续性 HPV7/9 种感染的风险。在 74 名 HPV 阳性男性中，71.6%的人清除了感染，实际和粗略的清除时间为 1.4 至 79.6 个月。没有发现 HPV7/9 清除的独立预测因素。在最后一次随访时，50.1%的父亲有口腔黏膜改变，仅与吸烟相关（p=0.046）。总之，男性中大多数持续性口腔感染是由 HPV16 引起的。吸烟增加，而生殖器疣减少则降低了口腔 HR-HPV 的持续性。未发现 HR-HPV 清除的预测因素。
