Guo Jinying, Shi Mingke, Zhao Yanli, Ren Guoyan, Yi Junpeng, Niu Leilei, Li Juan
Food & Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, China.
Wei Sheng Wu Xue Bao. 2013 Jun 4;53(6):553-60.
This study aimed to investigate the effects of Cu2+, Cr2+ and Pb2+ stress on Nostoc flagelliforme cell.
The response of Nostoc flagelliforme cell was analyzed under the stress. The modified BG11 culture medium containing different heavy metal ions of 0, 0.1, 1.0, 10, 100 mg/L was used to cultivate Nostoc flagelliforme cell at 25 degrees C and light intensity of 80 micromol/(m x s). Electrolyte leakage, the activities of superoxide dismutase, the content of malondialdehyde, proline, soluble protein and trehalose were analyzed.
Under 1 - 100 mg/L Cu2+, Cr2+ and Pb2+ stress, electrolyte leakage and malondialdehyde contents in Nostoc flagelliforme cell were higher than those in the control group during heavy metal ions stress. Meanwhile, superoxide dismutase activity increased slightly under 10 mg/L, but was lower afterwards. The contents of proline, soluble protein and trehalose increased under 10 mg/L heavy metal ions stress, while declined under extreme heavy metal ions stress (100 mg/L).
Nostoc flagelliforme cell has resistance to low heavy metal ions stress, but is damaged badly under extreme heavy metal ions stress.
本研究旨在探究Cu2+、Cr2+和Pb2+胁迫对发菜细胞的影响。
分析发菜细胞在胁迫下的反应。使用含有0、0.1、1.0、10、100 mg/L不同重金属离子的改良BG11培养基，在25℃、光照强度80微摩尔/（米·秒）的条件下培养发菜细胞。分析电解质渗漏、超氧化物歧化酶活性、丙二醛、脯氨酸、可溶性蛋白和海藻糖的含量。
在1 - 100 mg/L的Cu2+、Cr2+和Pb2+胁迫下，重金属离子胁迫期间发菜细胞的电解质渗漏和丙二醛含量高于对照组。同时，超氧化物歧化酶活性在10 mg/L时略有增加，但之后降低。脯氨酸、可溶性蛋白和海藻糖的含量在10 mg/L重金属离子胁迫下增加，而在极端重金属离子胁迫（100 mg/L）下下降。
发菜细胞对低浓度重金属离子胁迫具有抗性，但在极端重金属离子胁迫下会受到严重损害。
