Tang Hua, Liu Xinsheng, Fang Yuzhen, Jiang Shoutian, Pan Li, Lv Jianliang, Zhang Zhongwang, Zhou Peng, Zhang Yongguang, Wang Yonglu
State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Wei Sheng Wu Xue Bao. 2013 Jun 4;53(6):596-607.
We developed a synthetic vaccine against foot-and-mouth disease type A.
We studied two peptide-based vaccines containing residues 131 to 159 of VP1, 20 to 35 of VP4, 21 to 35 of 3A and 29 to 42 of 3B of the AF/72 strain of foot-and-mouth disease virus (FMDV) coupled with a CpG oligodeoxynucleotide (5'-TCGCGAACGTTCGCCCGATCGTCGGTA-3') in guinea pigs. We assayed the FMDV-specific IgG level, serum neutralizing antibody titer, splenic lymphocytes proliferative capacity and peripheral blood T lymphocyte CD4-CD8 subsets distribution.
The data show that high dose did not ensure a good immunity. In our study, 8% (4/5) of peptide 364-2.5-inoculated guinea pigs (2.5 microg of peptide 364 per animal) were protected against AF/72 strain challenge, while the protection ratio from other peptide-immunized groups was lower except the inactivated vaccine-inoculated group which showed a full protection. Our results also indicated that the stimulatory ability of CD4+ T lymphocyte response played a key role in evaluating effective FMDV vaccine. The highest percentage of CD4+ T lymphocyte was 36.6% appeared in inactivated vaccine-immunized guinea pigs, the second was 33.7% in peptide 364-2.5-vaccinated group, whereas the remaining ranged from 18.1% to 27.7%. There was no obvious relation between CD8+ T cells and anti-FMDV infection; our data showed that the CD4/CD8 ratio was not always appropriate for assessing the immune system status.
In general, we not only designed an effective vaccine against FMDV type A, but also discovered some useful information of humoral and cellular responses induced by foot-and-mouth disease vaccines.
我们研发了一种针对A型口蹄疫的合成疫苗。
我们研究了两种基于肽的疫苗，其包含口蹄疫病毒（FMDV）AF/72株VP1的131至159位残基、VP4的20至35位残基、3A的21至35位残基以及3B的29至42位残基，并与一种CpG寡脱氧核苷酸（5'-TCGCGAACGTTCGCCCGATCGTCGGTA-3'）偶联，在豚鼠中进行实验。我们检测了FMDV特异性IgG水平、血清中和抗体滴度、脾淋巴细胞增殖能力以及外周血T淋巴细胞CD4-CD8亚群分布。
数据表明高剂量并不能确保良好的免疫效果。在我们的研究中，接种肽364-2.5的豚鼠（每只动物2.5微克肽364）中有8%（4/5）受到保护，免受AF/72株攻击，而其他肽免疫组的保护率较低，除了接种灭活疫苗的组显示出完全保护。我们的结果还表明，CD4+T淋巴细胞反应的刺激能力在评估有效的FMDV疫苗中起关键作用。CD4+T淋巴细胞的最高百分比出现在接种灭活疫苗的豚鼠中，为36.6%，其次是接种肽364-2.5的组，为33.7%，而其余组的范围在18.1%至27.7%之间。CD8+T细胞与抗FMDV感染之间没有明显关系；我们的数据表明，CD4/CD8比率并不总是适合评估免疫系统状态。
总体而言，我们不仅设计了一种有效的针对A型FMDV的疫苗，还发现了一些由口蹄疫疫苗诱导的体液和细胞反应的有用信息。
