VTT Technical Research Centre of Finland , P.O. Box 1000, Tietotie 2, Espoo, Finland.
J Agric Food Chem. 2013 Oct 9;61(40):9744-53. doi: 10.1021/jf4024195. Epub 2013 Sep 26.
Brewer's spent grain (BSG), the major side-stream from brewing, is rich in protein, lignin, and nonstarch polysaccharides. Lignin is a polyphenolic macromolecule considered resilient toward breakdown and utilization by colon microbiota, although some indications of release of small phenolic components from lignin in animals have been shown. The aim of this study was to investigate if the human intestinal microbiota can release lignans and small phenolic compounds from whole BSG, a lignin-enriched insoluble fraction from BSG and a deferuloylated fraction, in a metabolic in vitro colon model. The formation of short-chain fatty acid (SCFA) was also investigated. More lignin-related monomers and dilignols were detected from the lignin-enriched fraction than from BSG or deferuloylated BSG. SCFA formation was not suppressed by any of the fractions. It was shown that small lignin-like compounds were released from these samples in the in vitro colon model, originating most likely from lignin.
啤酒糟（BSG）是酿造过程中的主要副产物，富含蛋白质、木质素和非淀粉多糖。木质素是一种多酚类大分子，被认为不易被结肠微生物群分解和利用，但有一些迹象表明木质素在动物体内会释放出一些小的酚类成分。本研究旨在探讨人类肠道微生物群是否能从整个 BSG、BSG 中的木质素富集不溶性部分以及去阿魏酰 BSG 中释放出木脂素和小酚类化合物，并在代谢体外结肠模型中进行研究。还研究了短链脂肪酸（SCFA）的形成。从木质素富集部分检测到的与木质素相关的单体和二聚体比 BSG 或去阿魏酰 BSG 多。任何部分都没有抑制 SCFA 的形成。结果表明，这些样品在体外结肠模型中释放出了小分子木质素样化合物，这些化合物很可能来自木质素。
