Laboratory of Microbial Ecology and Technology (LabMET), Ghent University , Coupure Links 653, 9000 Ghent, Belgium ;
J Agric Food Chem. 2013 Oct 16;61(41):9819-27. doi: 10.1021/jf4021784. Epub 2013 Oct 4.
Different in vitro models have been developed to assess how food compounds affect the human gut microbiota. Using two such models (SHIME(R) and TIM-2), we compared how long-chain arabinoxylan (LC-AX), a wheat-derived potentially prebiotic fiber, and inulin (IN), a well-established prebiotic compound, modulate SCFA production and bifidobacteria composition. While both the SHIME and TIM-2 differ in experimental design, they both demonstrated that LC-AX and IN specifically increased the health-promoting metabolites propionate and butyrate, respectively. Furthermore, LC-AX stimulated Bifidobacterium longum, while IN stimulated other bifidobacteria including Bifidobacterium adolescentis. The SHIME experiment also revealed that effects of LC-AX were more persistent during the 2-week wash-out period. These results confirm a recent in vivo study, during which humanized rats were treated with the same LC-AX/IN. In conclusion, results from different human gut models suggest that, besides IN, LC-AX are promising prebiotic candidates with high specificity toward Bifidobacterium longum and a selective propionate increase.
不同的体外模型已被开发出来，以评估食物化合物如何影响人类肠道微生物群。使用两种这样的模型（SHIME(R)和 TIM-2），我们比较了长链阿拉伯木聚糖（LC-AX），一种源自小麦的潜在益生元纤维，和菊粉（IN），一种成熟的益生元化合物，如何调节 SCFA 产生和双歧杆菌组成。虽然 SHIME 和 TIM-2 在实验设计上有所不同，但它们都表明 LC-AX 和 IN 分别特异性地增加了健康促进代谢物丙酸和丁酸。此外，LC-AX 刺激长双歧杆菌，而 IN 刺激其他双歧杆菌，包括青春双歧杆菌。SHIME 实验还表明，LC-AX 的作用在 2 周的冲洗期内更持久。这些结果证实了最近的一项体内研究，在该研究中，用人源化大鼠进行了相同的 LC-AX/IN 处理。总之，来自不同的人类肠道模型的结果表明，除了 IN，LC-AX 是有前途的益生元候选物，对长双歧杆菌具有高度特异性，并选择性地增加丙酸。
