Jeon Su Yeon, Park Ji Sun, Yang Han Na, Woo Dae Gyun, Park Keun-Hong
1 Department of Biomedical Science, College of Life Science, CHA University , Seongnam-si, Republic of Korea.
Stem Cells Dev. 2014 Feb 1;23(3):305-17. doi: 10.1089/scd.2013.0311. Epub 2013 Oct 19.
During embryogenesis, specific proteins expressed in cells have key roles in the formation of differentiated cells and tissues. Delivery of specific proteins into specific cells, both in vitro and in vivo, has proved to be exceedingly difficult. In this study, we developed a safe and efficient protein delivery system using encapsulation of proteins into biodegradable poly-(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). The PLGA NPs were used to deliver proteins into human mesenchymal stem cells (hMSCs). Fluorescent markers loaded into the PLGA NPs were used to verify the internalization of NPs into hMSCs using FACS analysis and confocal microscopy. With these methods, we demonstrated that the encapsulated model proteins are readily delivered into hMSCs, released from the NP vehicles, and, finally, moved into the cytosols. Using chondrogenesis-related proteins such as aggrecan and cartilage oligomeric matrix protein (COMP), chondrogenic differentiation of hMSCs treated with aggrecan and COMP encapsulated PLGA NPs was clearly observed and caused to differentiate into chondrocytes.
在胚胎发生过程中，细胞中表达的特定蛋白质在分化细胞和组织的形成中起关键作用。事实证明，将特定蛋白质递送至体外和体内的特定细胞都极其困难。在本研究中，我们开发了一种安全高效的蛋白质递送系统，该系统通过将蛋白质封装到可生物降解的聚（乳酸-乙醇酸）（PLGA）纳米颗粒（NP）中来实现。PLGA纳米颗粒被用于将蛋白质递送至人间充质干细胞（hMSC）。加载到PLGA纳米颗粒中的荧光标记物用于通过流式细胞术分析和共聚焦显微镜来验证纳米颗粒内化进入hMSC。通过这些方法，我们证明了封装的模型蛋白很容易被递送至hMSC，从NP载体中释放出来，并最终进入细胞质。使用诸如聚集蛋白聚糖和软骨寡聚基质蛋白（COMP）等与软骨形成相关的蛋白质，明显观察到用聚集蛋白聚糖和COMP封装的PLGA纳米颗粒处理的hMSC发生软骨形成分化，并使其分化为软骨细胞。
