Laboratory of Cardiovascular Research, Centre de Recherche Public de la Santé, 84 Val Fleuri, Luxembourg L1526, Luxembourg.
EJNMMI Res. 2013 Sep 12;3(1):65. doi: 10.1186/2191-219X-3-65.
Adenosine may have beneficial effects on left ventricular function after myocardial infarction (MI), but the magnitude of this effect on remote and MI areas is controversial. We assessed the long-term effects of adenosine after MI using electrocardiogram-triggered 18 F-fluorodeoxyglucose positron emission tomography.
Wistar rats were subjected to coronary ligation and randomized into three groups treated daily for 2 months by NaCl (control; n = 7), 2-chloroadenosine (CADO; n = 8) or CADO with 8-sulfophenyltheophilline, an antagonist of adenosine receptors (8-SPT; n = 8).
After 2 months, control rats exhibited left ventricular remodelling, with increased end-diastolic volume and decreased ejection fraction. Left ventricular remodelling was not significantly inhibited by CADO. Segmental contractility, as assessed by the change in myocardial thickening after 2 months, was improved in CADO rats compared to control rats (+1.6% ± 0.8% vs. -2.3% ± 0.8%, p < 0.001). This improvement was significant in border (+5.6% ± 0.8% vs. +1.5% ± 0.8%, p < 0.001) and remote (-4.0% ± 1.0% vs. -10.4% ± 1.3%, p < 0.001) segments, but absent in MI segments. Histological analyses revealed that CADO reduced fibrosis, cardiomyocyte hypertrophy and apoptosis. Protective effects of CADO were blunted by 8-SPT.
Long-term administration of adenosine protects the left ventricle from contractile dysfunction following MI.
腺苷可能对心肌梗死后左心室功能有益，但它对远隔和 MI 区的影响程度存在争议。我们使用心电图触发的 18F-氟脱氧葡萄糖正电子发射断层扫描评估了 MI 后腺苷的长期影响。
Wistar 大鼠接受冠状动脉结扎，并随机分为三组，每天接受 2 个月的 NaCl（对照组，n=7）、2-氯腺苷（CADO，n=8）或 CADO 加腺苷受体拮抗剂 8-磺基茶碱（8-SPT，n=8）治疗。
2 个月后，对照组大鼠出现左心室重构，表现为舒张末期容积增加和射血分数降低。CADO 对左心室重构没有明显抑制作用。2 个月后，通过心肌增厚变化评估节段收缩力，CADO 大鼠较对照组大鼠有所改善（+1.6%±0.8%对-2.3%±0.8%，p<0.001）。在边界（+5.6%±0.8%对+1.5%±0.8%，p<0.001）和远隔（-4.0%±1.0%对-10.4%±1.3%，p<0.001）节段，这种改善是显著的，但在 MI 节段不存在。组织学分析显示，CADO 减少了纤维化、心肌细胞肥大和细胞凋亡。8-SPT 减弱了 CADO 的保护作用。
长期给予腺苷可保护左心室免受 MI 后收缩功能障碍的影响。
