• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

腺苷能刺激人内皮祖细胞的迁移。CXCR4 和 microRNA-150 的作用。

Adenosine stimulates the migration of human endothelial progenitor cells. Role of CXCR4 and microRNA-150.

机构信息

Laboratory of Cardiovascular Research, Public Research Centre-Health (CRP-Santé), Luxembourg, Luxembourg.

出版信息

PLoS One. 2013;8(1):e54135. doi: 10.1371/journal.pone.0054135. Epub 2013 Jan 9.

DOI:10.1371/journal.pone.0054135
PMID:23326587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3541240/
Abstract

BACKGROUND

Administration of endothelial progenitor cells (EPC) represents a promising option to regenerate the heart after myocardial infarction, but is limited because of low recruitment and engraftment in the myocardium. Mobilization and migration of EPC are mainly controlled by stromal cell-derived factor 1α (SDF-1α) and its receptor CXCR4. We hypothesized that adenosine, a cardioprotective molecule, may improve the recruitment of EPC to the heart.

METHODS

EPC were obtained from peripheral blood mononuclear cells of healthy volunteers. Expression of chemokines and their receptors was evaluated using microarrays, quantitative PCR, and flow cytometry. A Boyden chamber assay was used to assess chemotaxis. Recruitment of EPC to the infarcted heart was evaluated in rats after permanent occlusion of the left anterior descending coronary artery.

RESULTS

Microarray analysis revealed that adenosine modulates the expression of several members of the chemokine family in EPC. Among these, CXCR4 was up-regulated by adenosine, and this result was confirmed by quantitative PCR (3-fold increase, P<0.001). CXCR4 expression at the cell surface was also increased. This effect involved the A(2B) receptor. Pretreatment of EPC with adenosine amplified their migration towards recombinant SDF-1α or conditioned medium from cardiac fibroblasts. Both effects were abolished by CXCR4 blocking antibodies. Adenosine also increased CXCR4 under ischemic conditions, and decreased miR-150 expression. Binding of miR-150 to the 3' untranslated region of CXCR4 was verified by luciferase assay. Addition of pre-miR-150 blunted the effect of adenosine on CXCR4. Administration of adenosine to rats after induction of myocardial infarction stimulated EPC recruitment to the heart and enhanced angiogenesis.

CONCLUSION

Adenosine increases the migration of EPC. The mechanism involves A(2B) receptor activation, decreased expression of miR-150 and increased expression of CXCR4. These results suggest that adenosine may be used to enhance the capacity of EPC to revascularize the ischemic heart.

摘要

背景

内皮祖细胞（EPC）的给药代表了一种有前途的心肌梗死后心脏再生的选择，但由于其在心肌中的募集和植入有限。EPC 的动员和迁移主要由基质细胞衍生因子 1α（SDF-1α）及其受体 CXCR4 控制。我们假设，作为一种心脏保护分子的腺苷可能改善 EPC 向心脏的募集。

方法

从健康志愿者的外周血单核细胞中获得 EPC。使用微阵列、定量 PCR 和流式细胞术评估趋化因子及其受体的表达。使用 Boyden 室测定法评估趋化性。在大鼠左前降支冠状动脉永久性闭塞后，评估 EPC 向梗死心脏的募集。

结果

微阵列分析显示，腺苷调节 EPC 中趋化因子家族的几个成员的表达。其中，腺苷上调 CXCR4，这一结果通过定量 PCR 得到证实（增加 3 倍，P<0.001）。CXCR4 细胞表面表达也增加。这种作用涉及 A（2B）受体。EPC 用腺苷预处理可增强其向重组 SDF-1α或心脏成纤维细胞条件培养基的迁移。这两种作用均被 CXCR4 阻断抗体所阻断。腺苷在缺血条件下也增加了 CXCR4，同时降低了 miR-150 的表达。通过荧光素酶测定证实了 miR-150 与 CXCR4 的 3'非翻译区结合。添加前 miR-150 削弱了腺苷对 CXCR4 的作用。在诱导心肌梗死后向大鼠给予腺苷可刺激 EPC 向心脏募集并增强血管生成。

结论

腺苷增加 EPC 的迁移。其机制涉及 A（2B）受体激活、miR-150 表达降低和 CXCR4 表达增加。这些结果表明，腺苷可用于增强 EPC 使缺血心脏再血管化的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/e052dc01d623/pone.0054135.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/0dbc4c7b6a21/pone.0054135.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/595aab5fb815/pone.0054135.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/7d7bde1fff9f/pone.0054135.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/3ecc3c4b8f5b/pone.0054135.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/f1fc3f950d02/pone.0054135.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/7f9a3d304fbb/pone.0054135.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/e052dc01d623/pone.0054135.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/0dbc4c7b6a21/pone.0054135.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/595aab5fb815/pone.0054135.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/7d7bde1fff9f/pone.0054135.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/3ecc3c4b8f5b/pone.0054135.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/f1fc3f950d02/pone.0054135.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/7f9a3d304fbb/pone.0054135.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d253/3541240/e052dc01d623/pone.0054135.g007.jpg

相似文献

1
Adenosine stimulates the migration of human endothelial progenitor cells. Role of CXCR4 and microRNA-150.腺苷能刺激人内皮祖细胞的迁移。CXCR4 和 microRNA-150 的作用。
PLoS One. 2013;8(1):e54135. doi: 10.1371/journal.pone.0054135. Epub 2013 Jan 9.
2
Rehmannia glutinosa extract activates endothelial progenitor cells in a rat model of myocardial infarction through a SDF-1 α/CXCR4 cascade.地黄提取物通过 SDF-1α/CXCR4 级联反应激活心肌梗死后大鼠模型中的内皮祖细胞。
PLoS One. 2013;8(1):e54303. doi: 10.1371/journal.pone.0054303. Epub 2013 Jan 18.
3
Danshensu accelerates angiogenesis after myocardial infarction in rats and promotes the functions of endothelial progenitor cells through SDF-1α/CXCR4 axis.丹参素通过 SDF-1α/CXCR4 轴促进大鼠心肌梗死后血管生成和内皮祖细胞功能。
Eur J Pharmacol. 2017 Nov 5;814:274-282. doi: 10.1016/j.ejphar.2017.08.035. Epub 2017 Aug 31.
4
Overexpression of SDF-1α enhanced migration and engraftment of cardiac stem cells and reduced infarcted size via CXCR4/PI3K pathway.SDF-1α 的过表达通过 CXCR4/PI3K 通路增强了心脏干细胞的迁移和植入，并减少了梗死面积。
PLoS One. 2012;7(9):e43922. doi: 10.1371/journal.pone.0043922. Epub 2012 Sep 11.
5
17β-Estradiol enhances the recruitment of bone marrow-derived endothelial progenitor cells into infarcted myocardium by inducing CXCR4 expression.17β-雌二醇通过诱导 CXCR4 表达增强骨髓源性内皮祖细胞向梗死心肌的募集。
Int J Cardiol. 2013 Jan 10;162(2):100-6. doi: 10.1016/j.ijcard.2011.05.074. Epub 2011 Jun 1.
6
The spleen recruits endothelial progenitor cell via SDF-1/CXCR4 axis in mice.在小鼠中，脾脏通过SDF-1/CXCR4轴募集内皮祖细胞。
J Recept Signal Transduct Res. 2010 Aug;30(4):246-54. doi: 10.3109/10799893.2010.488241.
7
Over-expression of CXCR4 on mesenchymal stem cells augments myoangiogenesis in the infarcted myocardium.间充质干细胞上CXCR4的过表达增强梗死心肌中的肌血管生成。
J Mol Cell Cardiol. 2008 Feb;44(2):281-92. doi: 10.1016/j.yjmcc.2007.11.010. Epub 2007 Dec 7.
8
Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction.骨源巢蛋白阳性间充质干细胞通过梗死心肌后募集心脏内皮细胞改善心功能。
Stem Cell Res Ther. 2019 Apr 27;10(1):127. doi: 10.1186/s13287-019-1217-x.
9
Spliced stromal cell-derived factor-1α analog stimulates endothelial progenitor cell migration and improves cardiac function in a dose-dependent manner after myocardial infarction.拼接的基质细胞衍生因子-1α类似物以剂量依赖的方式刺激内皮祖细胞迁移，并改善心肌梗死后的心脏功能。
J Thorac Cardiovasc Surg. 2010 Nov;140(5):1174-80. doi: 10.1016/j.jtcvs.2010.08.012.
10
Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice.CXCR4 预激活的内皮祖细胞输注可减轻 db/db 糖尿病小鼠的脑缺血损伤并促进修复。
PLoS One. 2012;7(11):e50105. doi: 10.1371/journal.pone.0050105. Epub 2012 Nov 21.

引用本文的文献

1
MicroRNA: Crucial modulator in purinergic signalling involved diseases.微小 RNA：参与疾病的嘌呤能信号中的关键调节因子。
Purinergic Signal. 2023 Mar;19(1):329-341. doi: 10.1007/s11302-022-09840-y. Epub 2022 Feb 2.
2
Current Adenosinergic Therapies: What Do Cancer Cells Stand to Gain and Lose?当前的腺苷能疗法：癌细胞将何去何从？
Int J Mol Sci. 2021 Nov 22;22(22):12569. doi: 10.3390/ijms222212569.
3
Differential effect of adenosine on rhabdomyosarcoma migration and proliferation.腺苷对横纹肌肉瘤迁移和增殖的差异作用。

本文引用的文献

1
Monocyte chemotactic protein 3 is a homing factor for circulating angiogenic cells.单核细胞趋化蛋白 3 是循环血管生成细胞的归巢因子。
Cardiovasc Res. 2012 Jun 1;94(3):519-25. doi: 10.1093/cvr/cvs140. Epub 2012 Apr 4.
2
microRNA-150 regulates mobilization and migration of bone marrow-derived mononuclear cells by targeting Cxcr4.microRNA-150 通过靶向 Cxcr4 调节骨髓源性单核细胞的动员和迁移。
PLoS One. 2011;6(10):e23114. doi: 10.1371/journal.pone.0023114. Epub 2011 Oct 19.
3
Proof-of-principle investigation of an algorithmic model of adenosine-mediated angiogenesis.
Arch Med Sci. 2018 Apr 26;16(2):414-427. doi: 10.5114/aoms.2018.75506. eCollection 2020.
4
MicroRNA-221 Is Cardioprotective and Anti-fibrotic in a Rat Model of Myocardial Infarction.微小RNA-221在心肌梗死大鼠模型中具有心脏保护和抗纤维化作用。
Mol Ther Nucleic Acids. 2019 Sep 6;17:185-197. doi: 10.1016/j.omtn.2019.05.018. Epub 2019 Jun 4.
5
No Differences in Levels of Circulating Progenitor Endothelial Cells or Circulating Endothelial Cells Among Patients Treated With Ticagrelor Compared With Clopidogrel During Non- ST -Segment-Elevation Myocardial Infarction.在非 ST 段抬高型心肌梗死患者中，与氯吡格雷相比，使用替格瑞洛治疗并未导致循环祖细胞内皮细胞或循环内皮细胞水平的差异。
J Am Heart Assoc. 2018 Oct 2;7(19):e009444. doi: 10.1161/JAHA.118.009444.
6
MicroRNA 150-5p Improves Risk Classification for Mortality within 90 Days after Acute Ischemic Stroke.微小RNA 150-5p改善急性缺血性卒中后90天内死亡风险分类
J Stroke. 2017 Sep;19(3):323-332. doi: 10.5853/jos.2017.00423. Epub 2017 Sep 29.
7
Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia.肺损伤途径：腺苷受体2B信号传导限制闭塞性细支气管炎伴机化性肺炎的发展。
Exp Lung Res. 2017 Feb;43(1):38-48. doi: 10.1080/01902148.2017.1286697. Epub 2017 Mar 7.
8
Endothelial Progenitor Cells Physiology and Metabolic Plasticity in Brain Angiogenesis and Blood-Brain Barrier Modeling.内皮祖细胞在脑微血管生成和血脑屏障建模中的生理学及代谢可塑性
Front Physiol. 2016 Dec 1;7:599. doi: 10.3389/fphys.2016.00599. eCollection 2016.
9
A comparison of microRNA expression profiles from splenic hemangiosarcoma, splenic nodular hyperplasia, and normal spleens of dogs.犬脾脏血管肉瘤、脾脏结节性增生和正常脾脏中微小RNA表达谱的比较。
BMC Vet Res. 2016 Dec 3;12(1):272. doi: 10.1186/s12917-016-0903-5.
10
MiR-150 Regulates Poststroke Cerebral Angiogenesis via Vascular Endothelial Growth Factor in Rats.miR-150 通过血管内皮生长因子调控大鼠卒中后血管新生。
CNS Neurosci Ther. 2016 Jun;22(6):507-17. doi: 10.1111/cns.12525. Epub 2016 Feb 28.
腺苷介导血管生成算法模型的原理验证研究
Theor Biol Med Model. 2011 Apr 8;8:7. doi: 10.1186/1742-4682-8-7.
4
Adenosine modifies the balance between membrane and soluble forms of Flt-1.腺苷调节 Flt-1 的膜型和可溶性形式之间的平衡。
J Leukoc Biol. 2011 Jul;90(1):199-204. doi: 10.1189/jlb.0910505. Epub 2011 Mar 29.
5
Predictive integration of gene functional similarity and co-expression defines treatment response of endothelial progenitor cells.基因功能相似性与共表达的预测性整合确定了内皮祖细胞的治疗反应。
BMC Syst Biol. 2011 Mar 30;5:46. doi: 10.1186/1752-0509-5-46.
6
Stem and progenitor cell-based therapy in ischaemic heart disease: promise, uncertainties, and challenges.基于干细胞和祖细胞的治疗缺血性心脏病：前景、不确定性和挑战。
Eur Heart J. 2011 May;32(10):1197-206. doi: 10.1093/eurheartj/ehr018. Epub 2011 Feb 28.
7
International Union of Basic and Clinical Pharmacology. LXXXI. Nomenclature and classification of adenosine receptors--an update.国际基础和临床药理学联合会. LXXXI. 腺苷受体的命名和分类-更新。
Pharmacol Rev. 2011 Mar;63(1):1-34. doi: 10.1124/pr.110.003285. Epub 2011 Feb 8.
8
Forecasting the future of cardiovascular disease in the United States: a policy statement from the American Heart Association.预测美国心血管疾病的未来：美国心脏协会的政策声明。
Circulation. 2011 Mar 1;123(8):933-44. doi: 10.1161/CIR.0b013e31820a55f5. Epub 2011 Jan 24.
9
Platelet microparticles enhance the vasoregenerative potential of angiogenic early outgrowth cells after vascular injury.血小板微粒增强血管损伤后促血管生成早期外生细胞的血管再生潜能。
Circulation. 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473. Epub 2010 Jul 19.
10
Secreted monocytic miR-150 enhances targeted endothelial cell migration.分泌型单核细胞 miR-150 增强靶向性内皮细胞迁移。
Mol Cell. 2010 Jul 9;39(1):133-44. doi: 10.1016/j.molcel.2010.06.010.